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NSJ/ABCA1 Antibody (R30941)/ R30941/ 100 ug

Formulation:

0.5mg/mlifreconstitutedwith0.2mlsterileDIwater

Data:

AvailABIlity1-3businessdays
SpeciesReactivityHuman,Mouse,Rat
FormatAntigenaffinitypurified
ClonalityPolyclonal(rabbitorigin)
IsotypeRabbitIgG
PurityAntigenaffinity
BufferLyophilizedfrom1XPBSwith2.5%BSAand0.025%sodiumazide/thimerosal
UniProtO95477
ApplicationsWesternblot:0.5-1ug/ml
LimitationsThisABCA1antibodyisavailableforresearchuseonly.

Description:

ATP-bindingcassette,sub-familyA,member1,alsoknownasABC1,andtheCholesteroleffluxregulatoryprotein(CERP),isaproteinwhichinhumansisencodedbytheABCA1gene.Themembrane-associatedproteinencodedbythisgeneisamemberofthesuperfamilyofATP-bindingcassette(ABC)transporters.ABCproteinstransportvariousmoleculesacrossextra-andintracellularmembranes.Dotblotanalysisof50tissuesrevealedubiquitousexpressionofABCA1mRNA,withhighestexpressioninplacenta,liver,lung,adrenalglands,andallfetaltissuesexamined,andlowestexpressioninkidney,pancreas,pituitary,mammarygland,andbonemarrow.ThisproteinisamemberoftheABCAsubfamily.MembersoftheABCAsubfamilycomprisetheonlymajorABCsubfamilyfoundexclusivelyinmulticellulareukaryotes.Withcholesterolasitssubstrate,thisproteinfunctionsasacholesteroleffluxpumpinthecellularlipidremovalpathway.UsinghumanABCA1expressedinthemembranefractionofsf9insectcells,Szakacsetal.foundspecific,Mg(2+)-dependentATPbindingandlowbasalATPaseactivity.AdditionofpotentiallipidsubstratesorlipidacceptorsdidnotmodifytheATPaseactivityornucleotideocclusionbyABCA1.Szakacsetal.speculatedthatitmaybearegulatoryproteinormayrequireotherproteinpartnersforfullactivation.

ApplicationNotes:

Thestatedapplicationconcentrationsaresuggestedstartingamounts.TitrationoftheABCA1antibodymayberequiredduetodifferencesinprotocolsandsecondary/substratesensitivity.

Immunogen:

AnaminoacidsequencefromtheC-terminusofhumanABCA1(NFAKDQSDDDHLKDLSLH)wasusedastheimmunogenforthisABCA1antibody(100%homologousinhuman,mouseandrat).

Storage:

Afterreconstitution,theABCA1antibodycanbestoredforuptoonemonthat4oC.Forlong-term,aliquotandstoreat-20oC.Avoidrepeatedfreezingandthawing.
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