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The DIY (Do it Yourself) Elisa Kit H1 or DIYEK H1 (ref LIK-1101) is an immunoassay enables 80 samples in duplicate to be analyzed in two 96-well plates. Simply have 100 µL of serum, plasma or human cell culture supernatant to perform this assay.
This immunoassay, based on the principle of a sandwich ELISA (i.e. using two monoclonal anti-human Endocan antibodies) allows the quantification of Endocan in samples (supernatants culture, plasma or serum).
The DIYEK H1 (LIK-1101) contains the essential compounds to allow quantification of Endocan in biological fluids (serum, plasma, ascites fluid, vitreous humor, etc.) and in cell culture supernatants human :
The protocol (inserted into the kit and downloadable from our website) is easily achievable and provides highly reproducible results.
You can also buy Endocan calibrators independently according to your plate patterns (companion product: LIP-1101).
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn