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Lunginnov/Do It Yourself ELISA Kit H1 Bulk 5 plates (human Endocan)/LIK-1101B5

Description:

LIK-1101

TheDIY(DoitYourself)ELISAKitH1orDIYEKH1(refLIK-1101B5)isanimmunoassayenables200samplesinduplicatetobeanalyzedintwo96-wellplates.Simplyhave100µLofserum,plasmaorhumancellculturesupernatanttoperformthisassay.

Thisimmunoassay,basedontheprincipleofasandwichELISA(i.e.usingtwomonoclonalanti-humanEndocanantibodies)allowsthequantificationofEndocaninsamples(supernatantsculture,plasmaorserum).

TheDIYEKH1(LIK-1101B5)containstheessentialcompoundstoallowquantificationofEndocaninBIOLOGicalfluids(serum,plasma,ascitesfluid,vitreoushumor,etc.)andincellculturesupernatantshuman:

  • Onevialwithanti-humanEndocanmonoclonalantibody,captureantibody,
  • Onevialwithanti-humanmonoclonalantibodyEndocan,detectionantibody,
  • TwovialsoflyophilizedrecombinantEndocanforcalibration.

Theprotocol(insertedintothekitanddownloadablefromourwebsite)iseasilyachievableandprovideshighlyreproducIBLeresults.

YoucanalsobuyEndocancalibratorsindependentlyaccordingtoyourplatepatterns(companionproduct:LIP-1101).

Characteristics:

  • SandwichELISAusingtwomonoclonalantibodiespurifiedbyaffinity
  • DetectsthehumanandmonkeyEndocan
  • Format:quantitysufficientfor5x96wells
  • Duration:4hours
  • Linearity:0.4to10ng/mL
  • Detectionlimit:0.2ng/mL
  • Limitofquantification:0.4ng/mL
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