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Lunginnov/Do It Yourself Kit M1 ELISA (murine endocan)/LIK-1102

Description:

LIK-1102

TheDIY(DoitYourself)ELISAKitM1orDIYEKM1(refLIK-1102)isanimmunoassayenables88samplesinduplicatetobeanalyzedintwo96-wellplates.

Simplyhave100µLofserum,plasmaorculturesupernatantofmurinecellstoperformthisassay.

TheDIYEKM1(LIK-1102)containstheessentialcompoundstoallowforthequantificationofEndocaninBIOLOGicalfluids(serum,plasma,etc.)andintheculturesupernatants:

  • Onevialwithanti-humanEndocanmonoclonalantibody,captureantibody,
  • Onevialwithanti-humanmonoclonalantibodyEndocan,detectionantibody,
  • TwovialsoflyophilizedrecombinantmouseEndocanforquantification.

Theprotocol(insertedintothekitanddownloadablefromourwebsite)iseasilyachievableandprovideshighlyreproducIBLeresults.

YoucanalsobuythemouseEndocanindependentlyforyourplatepatterns(companionproduct:LIP-1104).

Characteristics:

  • SandwichELISAusingtwomonoclonalantibodiespurifiedbyaffinity
  • DetectsthehumanandmonkeyEndocan
  • Format:quantitysufficientfor2x96wells
  • Duration:4hours
  • Linearity:0.3to10ng/mL
  • Detectionlimit:0.3ng/ml
  • Limitofquantification:0.6ng/ml
  • InterSeriesreproducibility(%CV):9.19%
  • WithinrunrepeatABIlity(%CV):5.79%
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