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Lunginnov/Just Do It ELISA Kit H1 (human Endocan)/LIK-1205

Description:

LIK-1205

TheJDIEK(JustDoItELISAKit)H1isasandwichELISA(ieusingtwomonoclonalanti-humanEndocanantibodies)enables40samplestobeanalyzedinduplicate.

Simplyhave100µLofserum,plasmaorhumancellculturesupernatanttoperformthisassay.

TheJDIEKH1(LIK-1205)containsallofthecompoundstoallowforthequantificationofEndocaninBIOLOGicalfluids(serum,plasma,ascitesfluid,vitreoushumor,etc.)andinculturesupernatants:

  • One96-wellplatepre-coatedwithcaptureAntibody,
  • twovialsoflyophilizedrecombinanthumanEndocanforquantification(LIP-1101),
  • Onevialwithbiotin-coupled,anti-humanEndocanmonoclonalantibody,saiddetectionAntibody,
  • Onevialofenzymereagent,
  • OnevialofELISAbuffer20X,
  • Onevialofsubstratesolution,
  • Onevialoftheacidstopsolution,
  • TwoadhesivefilmsELISAplatesealing.

Theprotocol(insertedintothekitanddownloadablefromourwebsite)iseasilyachievableandprovideshighlyreproducIBLeresults.

YoucanalsobuyindependentlyofadditionalvialsoflyophilizedhumanEndocan(companionproduct:LIP-1101).

Characteristics:

  • DetectsthehumanandmonkeyEndocan
  • •Linearity:0.3to5ng/ml
  • •detectionlimit:0.15ng/ml
  • •Limitofquantification:0.3ng/ml
  • •WithinrunrepeatABIlity(%CV):4.80%
  • •InterSeriesreproducibility(%CV):7.59%
  • •Interference:Nointerferenceobservedwithhemolyzedplasma,milkyorjaundiced
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