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Gentarget/CRE-2A-GFP (Bsd), EF1a lentivirus/LVP525/1 Ea

Pre-madelentiviralparticlesbicistronicallyexpresstwoindividualproteins:nuclearpermeableCRE recombinaseandtheGFPMarkerunderthesameenahncedEf1apromoter.CREcontainsaNLS(nuclearlocalizationsignal) atitsN-term.This nuclearpermeant CRE increasestherecombinationeventsinvivo.ABlasticidinmarkerwasexpressedunderRSVpromoter.ParticlesallowstosortorselecttransducedcellsforlongtermexpressionviaGFPsignalorviablasticidinantibioticselection.

CRE protein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.PurifiedCREenzymecanjointindividualplasmidscontainingloxsites.

CREandGFPareconstitutivelyexpressedinhighlevel,indenpendentofcelltypes.GFPsignalprovidesaconvenient,realtimemonitoringthe particlesperformance.  Seeproductmanualfordetails(.pdf).

Amount:  200ul/pervial,at1x107IFU/mlinDMEMmediumwith10%FBSand10xpolybrene. 

Cat#:LVP525

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