Adipogen/Kdo2Lipid A 96well Plate (Sterile)/AG44T0002KI01/1 Plate

Specifications&Handling:

ProductData
Synonyms	Kdo2-LipidA-TLR4AgonistArray
Properties
ApplicationSet	CompoundScreening
Specificity	TLR4
OtherProductData	AdditionalStorageAdvise: Unopenedpouch:Ambient.Donotfreeze. Openedplatestoredinre-sealedpouch:4°C.Donotfreeze.Ifkeptsterile,theplate(withtheincludedlidattached)canbeusedforamultiplecustomizedexperimentalsetupswherecellculturesuspensionscanbeaddedsequentiallyorindividualwellsusedforseparatetestdates. Alreadyusedwellsshouldbemarkedappropriatelyandanycellculturemediumaspiratedcompletelyundersterileconditions.Anypartiallyusedplateshouldbekeptsterileuntilfurtheruse. ClickhereforfullProductDatasheet.
ProductType	Kit
ShippingandHandling
Shipping	AMBIENT
ShortTermStorage	+20°C
LongTermStorage	+20°C
HandlingAdvice	Donotfreeze.
Use/Stability	Stableforatleast2yearsafterreceiptwhenstoredat+20°C.
Documents
Manual	Download
MSDS	Download
RelatedProducts
MPLA(synthetic)96-wellPlate(Sterile) MPLAOn-The-Plate™(Sterile)(12x8Tests) LipidAOn-The-Plate™(Sterile)(12x8Tests) LPSOn-The-Plate™(Sterile)(12x8Tests)

Description:

Kdo2-LipidAplatescontainahighquality,sterileflat-bottomedclearpolystyrene96-wellcellcultureplatepre-coatedwithhighpurityLPSfromE.coli(Re)(Kdo2-LipidA).Theplatesarecoatedinauniform,stabilizedandactivelayerofpredefinedquantitiesofhighpurityKdo2-LipidAandtheyaresetupas12testcolumnswithidenticalagonistconcentrationsin7rows(A-G):
RowConcentrations:1μg(A),100ng(B),10ng(C),1ng(D),100pg(E),10pg(F),1pg(G)perwellandonenegativecontrolrow(H).

ARRAYPROCEDURE:
AddprimarycellsorcelllinesexpressingTLR4totheKdo2-LipidAplatewellscontainingthecoatedTLR4-selectivehighpurityKdo2-LipidA.Optimalcelldensityneedstobedeterminedfortherespectivecelltypeandreadout,recommendedstartingconcentrationis50,000macrophagesperwell.RecommendedcellculturevolumetobeaddedtotheKdo2-LipidAplateis100μl(200μl)per96-well,correspondingtoastartconcentrationof10μg/ml(5μg/ml)perwellfollowedby10-folddilutionsto10pg/ml(5pg/ml).

APPLICATION&CONVENIENCE:
1.Kdo2-LipidAplatesfacilitatethedeterminationoftheoptimalcelldensity,timepointofanalysisandmultiplexingofreadouts(e.g.cytokine(s)ELISA)ofTLR4specificcellularactivationinahighlyreproducibleandsafeprocedureexcludingthepossibilityofcontaminationorhazardoushandlingofKdo2-LipidA.
2.Kdo2-LipidAplatesareusefulforestablishingadoseresponsecurvespecificforthechosencelltypeandserumconcentrationandfortestingofTLR4inhibitorsorsensitizers.
3.Thisisaconvenientandtimesavingformatthathelpstoavoiddilutionerrors,partialsolubilisationorcontaminationwithKdo2-LipidA.