Adipogen/antiFOXP3 (mouse), mAb (MF333F)/AG20A0025C050/50 µg

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Product Details
Synonyms	Forkhead Box Protein P3; Scurfin
Product Type	Monoclonal Antibody
Properties
Clone	MF333F
Isotype	Rat IgG2aκ
Immunogen/Antigen	Recombinant mouse FOXP3.
Application	ELISA: (direct or indirect: 1:2000-1:10000) Immunocytochemistry Immunohistochemistry (1:50-1:100, frozen or paraffin sections) Western blot: (1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application.
Crossreactivity	Mouse
Specificity	Recognizes mouse FOXP3. Detects a band of ~46-49kDa by Western blot.
Purity Detail	Protein G-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

FOXP3 is involved in immune system responses. It functions as the master regulator in the development and function of regulatory T cells. FOX proteins belong to the forkhead/winged-helix family of transcriptional regulators and are presumed to exert control via similar DNA binding interactions during transcription. Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX); also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema.

Product References

Alternatively activated dendritic cells preferentially secrete IL-10, expand Foxp3+CD4+ T cells, and induce long-term organ allograft survival in combination with CTLA4-Ig: Y.Y. Lan, et al.; J. Immunol. 177, 5868 (2006)
Dendritic cells expressing transgenic galectin-1 delay onset of autoimmune diabetes in mice: M.J. Perone, et al.; J. Immunol. 177, 5278 (2006)
Immunosurveillance of Erbb2 carcinogenesis in transgenic mice is concealed by a dominant regulatory T-cell self-tolerance: E. Ambrosino, et al.; Cancer Res. 66, 7734 (2006)
Regulated compartmentalization of programmed cell death-1 discriminates CD4+CD25+ resting regulatory T cells from activated T cells: G. Raimondi, et al.; J. Immunol. 176, 2808 (2006)
Antimetastatic activity of a preventive cancer vaccine: P. Nanni, et al.; Cancer Res. 67, 11037 (2007)
Rapamycin-conditioned dendritic cells are poor stimulators of allogeneic CD4+ T cells, but enrich for antigen-specific Foxp3+ T regulatory cells and promote organ transplant tolerance: H.R. Turnquist, et al.; J. Immunol. 178, 7018 (2007)
Pretransplant infusion of mesenchymal stem cells prolongs the survival of a semiallogeneic heart transplant through the generation of regulatory T cells: F. Casiraghi, et al.; J. Immunol. 181, 3933 (2008)
Suppression of autoimmune diabetes by soluble galectin-1: M.J. Perone, et al.; J. Immunol. 182, 2641 (2009)
Protective role for TLR4 signaling in atherosclerosis progression as revealed by infection with a common oral pathogen: C. Hayashi, et al.; J. Immunol. 189, 3681 (2012)
Acceleration of diabetes development in CXC chemokine receptor 3 (CXCR3)-deficient NOD mice: Y. Yamada, et al.; Diabetologia 55, 2238 (2012)

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Adipogen/antiFOXP3 (mouse), mAb (MF333F)/AG20A0025C050/50 µg