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GenWay/Rat Alpha 2-Macroglobulin ELISA Kit/GWB-98EFFB/96 well188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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GenWay/Rat Alpha 2-Macroglobulin ELISA Kit/GWB-98EFFB/96 well

Description:

ShortDescription:InthisassaytheAlpha2-Macroglobulinpresentinserumsamplereactswiththeanti-A2M.antibodieswhichhavebeenadsorbedtothesurfaceofpolystyrenemicrotitrewells.Aftertheremovalofunboundserumproteinsbywashing,anti-A2M.antibodiesconjugatedwithhorserADIshperoxidase(HRP),areadded.Theseenzyme-labeledantibodiesformcomplexeswiththepreviouslyboundserumA2M.Followinganotherwashingstep,theenzymeboundtotheimmunosorbentisassayedbytheadditionofachromogenicsubstrate,3,3’,5,5’-tetramethylbenzidine(TMB).ThequantityofboundenzymevariesdirectlywiththeconcentrationofAlpha2-Macroglobulininthesampletested;thus,theabsorbance,at450nm,isameasureoftheconcentrationofAlpha2-Macroglobulininthetestsample.ThequantityofAlpha2-Macroglobulininthetestsamplecanbeinterpolatedfromthestandardcurveconstructedfromthestandards,andcorrectedforserumdilution.

AdditionalInformation:

NameRatAlpha2-MacroglobulinELISAKit
RelatedProductNamesALPHA2M.CPAMD5;RatAlpha2-MacroglobulinELISAKit;Alpha-2-M;C3andPZP-likealpha-2-macroglobulindomain-containingprotein5CPAMD5RatAlpha2-MacroglobulinA2M
AliasSymbolsA2M,Alpha-2-macroglobulin,CPAMD5
ProteinNamePregnancyzoneprotein
DescriptionofTargetIsabletoinhibitallfourclassesofproteinasesbyaunique"trapping"mechanism.Thisproteinhasapeptidestretch,calledthe"baitregion"whichcontainsspecificcleavagesitesfordifferentproteinases.Whenaproteinasecleavesthebaitregion,
SwissprotIDQ61838
SpeciesReactivityRat
AssayInfoAssayMethodology:QuantitativeSandwichELISA
Sensitivity2.330ng/ml
KitRange6.25ng/ml-400ng/ml
HomologyRat
NCBIAccNumberNP_000005.2
StandardRangeReferToManual
SampleTypeSerum,plasmaandotherBIOLOGicalfluids
ProductFormatInSolution
Specimen/VolumesReferToManual
SwissProtNumberP01023
ApplicationsELISA
ReconstitutionandStorage1.DILUENT
The5XDiluentConcentrateisstableuntiltheexpira-tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionsshouldbestoredat4-8C.
2.WASHSOLUTION
The20XWashSolutionConcentrateisstableuntiltheexpira¬tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionscanbestoredatroomtemperature(1625C)orat4-8C.
3.ENZYME-ANTIBODYCONJUGATE
Undilutedhorseradishperoxidaseanti-A2Mconjugateshouldbestoredat4-8Canddilutedimmediatelypriortouse.Theworkingconjugatesolutionisstableforupto8hours.
4.CHROMOGEN-SUBSTRATESOLUTION
TheSubstrateSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate.
5.STOPSOLUTION
TheStopSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate.
6.ANTI-RATALPHA2-MACROGLOBULINELISAMICROPLATE
Anti-RatA2Mcoatedwellsarestableuntiltheexpirationdate,andshouldbestoredat4-8Cinsealedfoilpouchwithdesiccantpack.
7.RATALPHA2-MACROGLOBULINCALIBRATOR
ThelyophilizedRatAlpha2-Macroglobulincalibratorshouldbestoredat4Corfrozenuntilreconstituted.Thereconstitutedcalibratorshouldbealiquotedoutandstoredfrozen(Avoidmultiplefreeze-thawcycles).Theworkingstandardsolutionsshouldbepreparedimmediatelypriortouseandarestableforupto8hours.
ReactivityRat
KitSize96well
KitPrincipleGenWayBiotechAlpha-2MacroglobulinELISAKit(Rat)(GWB-98EFFB)isbasedonstandardsandwichenzyme-linkedimmuno-sorbentassaytechnology.AnantibodyforPregnancyzoneproteinhasbeenpre-coatedontoa96-wellplate(12x8WellStrips).Standardsortestsamplesareaddedtothewells,incubatedandwashedtoremoveunboundproteins.AnAnti-Pregnancyzoneprotein-HRPconjugateddetectorantibodyisthenadded,incubatedandunboundconjugateiswashedaway.AnenzymaticreactionisproducedthroughtheadditionofTMBsubstratewhichiscatalyzedbyHRPgeneratingabluecolorproductthatchangestoyellowafteraddingacidicstopsolution.Thedensityofyellowcolorationreadbyabsorbanceat450nmisquantitativelyproportionaltotheamountofsamplePregnancyzoneproteincapturedinthewell.
KitComponent
ComponentAmount
Anti-RatAlpha-2MacroglobulinMicroplate96Wells(12x8WellStrips)
20XDiluentConcentrate50mL
20XWashSolution50mL
100XEnzyme-AntibodyConjugate150uL
Chromogen-SubstrateSolution12mL
StopSolution12mL
RatAlpha-2MacroglobulinCalibrator1
KitDuration80Minutes
StorageRefertokitinstructionsforcomponentstorage
LeadTimeDomestic:within1weekdelivery International:1week
IntendedUseResearchUseOnly
AdditionalInformationIntendedUse:TheAlpha2-Macroglobulintestkitsareahighlysensitivetwo-siteenzymelinkedimmunoassay(ELISA)formeasuringAlpha2-Macroglobulininbiologicalsamplesofrats.
::Reagents:
1.DILUENTCONCENTRATE(RunningBuffer)
Onebottlecontaining50mlofa5Xconcentrateddiluentrunningbuffer.
2.WASHSOLUTIONCONCENTRATE
Onebottlecontaining50mlofa20Xconcentratedwashsolution.
3.ENZYME-ANTIBODYCONJUGATE100X
Onevialcontaining150ulofaffinitypurifiedanti-RatAlpha2-MacroglobulinantibodyconjugatedwithhorseradishperoxidaseinastABIlizingbuffer.
4.CHROMOGEN-SUBSTRATESOLUTION
Onevialcontaining12mLof3,3’,5,5’-tetramethybenzidine(TMB)andhydrogenperoxideincitricacidbufferatpH3.3.
5.STOPSOLUTION
Onevialcontaining12ml0.3Msulfuricacid.
WARNING:Avoidcontactwithskin.
6.ANTI-RATALPHA2-MACROGLOBULINELISAMICROPLATE
Twelveremovableeight(8)wellmicrowellstripsinwellholderframe.Eachwelliscoatedwithaffinitypurifiedanti-RatA2M.
7.RATALPHA2-MACROGLOBULINCALIBRATOR
OnevialcontainingalyophilizedRatAlpha2-Macroglobulincalibrator.