Name | RatAlpha2-MacroglobulinELISAKit | ||||||||||||||||
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RelatedProductNames | ALPHA2M.CPAMD5;RatAlpha2-MacroglobulinELISAKit;Alpha-2-M;C3andPZP-likealpha-2-macroglobulindomain-containingprotein5CPAMD5RatAlpha2-MacroglobulinA2M | ||||||||||||||||
AliasSymbols | A2M,Alpha-2-macroglobulin,CPAMD5 | ||||||||||||||||
ProteinName | Pregnancyzoneprotein | ||||||||||||||||
DescriptionofTarget | Isabletoinhibitallfourclassesofproteinasesbyaunique"trapping"mechanism.Thisproteinhasapeptidestretch,calledthe"baitregion"whichcontainsspecificcleavagesitesfordifferentproteinases.Whenaproteinasecleavesthebaitregion, | ||||||||||||||||
SwissprotID | Q61838 | ||||||||||||||||
SpeciesReactivity | Rat | ||||||||||||||||
AssayInfo | AssayMethodology:QuantitativeSandwichELISA | ||||||||||||||||
Sensitivity | 2.330ng/ml | ||||||||||||||||
KitRange | 6.25ng/ml-400ng/ml | ||||||||||||||||
Homology | Rat | ||||||||||||||||
NCBIAccNumber | NP_000005.2 | ||||||||||||||||
StandardRange | ReferToManual | ||||||||||||||||
SampleType | Serum,plasmaandotherBIOLOGicalfluids | ||||||||||||||||
ProductFormat | InSolution | ||||||||||||||||
Specimen/Volumes | ReferToManual | ||||||||||||||||
SwissProtNumber | P01023 | ||||||||||||||||
Applications | ELISA | ||||||||||||||||
ReconstitutionandStorage | 1.DILUENT The5XDiluentConcentrateisstableuntiltheexpira-tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionsshouldbestoredat4-8C. 2.WASHSOLUTION The20XWashSolutionConcentrateisstableuntiltheexpira¬tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionscanbestoredatroomtemperature(1625C)orat4-8C. 3.ENZYME-ANTIBODYCONJUGATE Undilutedhorseradishperoxidaseanti-A2Mconjugateshouldbestoredat4-8Canddilutedimmediatelypriortouse.Theworkingconjugatesolutionisstableforupto8hours. 4.CHROMOGEN-SUBSTRATESOLUTION TheSubstrateSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate. 5.STOPSOLUTION TheStopSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate. 6.ANTI-RATALPHA2-MACROGLOBULINELISAMICROPLATE Anti-RatA2Mcoatedwellsarestableuntiltheexpirationdate,andshouldbestoredat4-8Cinsealedfoilpouchwithdesiccantpack. 7.RATALPHA2-MACROGLOBULINCALIBRATOR ThelyophilizedRatAlpha2-Macroglobulincalibratorshouldbestoredat4Corfrozenuntilreconstituted.Thereconstitutedcalibratorshouldbealiquotedoutandstoredfrozen(Avoidmultiplefreeze-thawcycles).Theworkingstandardsolutionsshouldbepreparedimmediatelypriortouseandarestableforupto8hours. | ||||||||||||||||
Reactivity | Rat | ||||||||||||||||
KitSize | 96well | ||||||||||||||||
KitPrinciple | GenWayBiotechAlpha-2MacroglobulinELISAKit(Rat)(GWB-98EFFB)isbasedonstandardsandwichenzyme-linkedimmuno-sorbentassaytechnology.AnantibodyforPregnancyzoneproteinhasbeenpre-coatedontoa96-wellplate(12x8WellStrips).Standardsortestsamplesareaddedtothewells,incubatedandwashedtoremoveunboundproteins.AnAnti-Pregnancyzoneprotein-HRPconjugateddetectorantibodyisthenadded,incubatedandunboundconjugateiswashedaway.AnenzymaticreactionisproducedthroughtheadditionofTMBsubstratewhichiscatalyzedbyHRPgeneratingabluecolorproductthatchangestoyellowafteraddingacidicstopsolution.Thedensityofyellowcolorationreadbyabsorbanceat450nmisquantitativelyproportionaltotheamountofsamplePregnancyzoneproteincapturedinthewell. | ||||||||||||||||
KitComponent |
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KitDuration | 80Minutes | ||||||||||||||||
Storage | Refertokitinstructionsforcomponentstorage | ||||||||||||||||
LeadTime | Domestic:within1weekdelivery International:1week | ||||||||||||||||
IntendedUse | ResearchUseOnly | ||||||||||||||||
AdditionalInformation | IntendedUse:TheAlpha2-Macroglobulintestkitsareahighlysensitivetwo-siteenzymelinkedimmunoassay(ELISA)formeasuringAlpha2-Macroglobulininbiologicalsamplesofrats. | ||||||||||||||||
:: | Reagents: 1.DILUENTCONCENTRATE(RunningBuffer) Onebottlecontaining50mlofa5Xconcentrateddiluentrunningbuffer. 2.WASHSOLUTIONCONCENTRATE Onebottlecontaining50mlofa20Xconcentratedwashsolution. 3.ENZYME-ANTIBODYCONJUGATE100X Onevialcontaining150ulofaffinitypurifiedanti-RatAlpha2-MacroglobulinantibodyconjugatedwithhorseradishperoxidaseinastABIlizingbuffer. 4.CHROMOGEN-SUBSTRATESOLUTION Onevialcontaining12mLof3,3’,5,5’-tetramethybenzidine(TMB)andhydrogenperoxideincitricacidbufferatpH3.3. 5.STOPSOLUTION Onevialcontaining12ml0.3Msulfuricacid. WARNING:Avoidcontactwithskin. 6.ANTI-RATALPHA2-MACROGLOBULINELISAMICROPLATE Twelveremovableeight(8)wellmicrowellstripsinwellholderframe.Eachwelliscoatedwithaffinitypurifiedanti-RatA2M. 7.RATALPHA2-MACROGLOBULINCALIBRATOR OnevialcontainingalyophilizedRatAlpha2-Macroglobulincalibrator. |