Name | RabbitCRP(C-ReactiveProtein)ELISAKit | ||||||||||||||||
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RelatedProductNames | RabbitCRP(C-ReactiveProtein)ELISAKit;N/APTX1RabbitCRP(C-ReactiveProtein)CRP | ||||||||||||||||
Geneid | 100009091 | ||||||||||||||||
AliasSymbols | CRP,PTX1 | ||||||||||||||||
ProteinName | C-reactiveprotein | ||||||||||||||||
DescriptionofTarget | Displaysseveralfunctionsassociatedwithhostdefense:itpromotesagglutination,bacterialcapsularswelling,phagocytosisandcomplementfixationthroughitscalcium-dependentbindingtophosphorylcholine.CaninteractwithDNAandhistonesandmaysc | ||||||||||||||||
SwissprotID | P02742 | ||||||||||||||||
SpeciesReactivity | Rabbit | ||||||||||||||||
AssayInfo | AssayMethodology:QuantitativeSandwichELISA | ||||||||||||||||
Sensitivity | 1.610ng/ml | ||||||||||||||||
KitRange | 6.25ng/ml-200ng/ml | ||||||||||||||||
Homology | Rabbit | ||||||||||||||||
NCBIAccNumber | NP_000558.2 | ||||||||||||||||
StandardRange | ReferToManual | ||||||||||||||||
SampleType | Serum,plasmaandotherBIOLOGicalfluids | ||||||||||||||||
ProductFormat | InSolution | ||||||||||||||||
Specimen/Volumes | ReferToManual | ||||||||||||||||
SwissProtNumber | P02741 | ||||||||||||||||
Applications | ELISA | ||||||||||||||||
ReconstitutionandStorage | 1.DILUENT The5XDiluentConcentrateisstableuntiltheexpira-tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionsshouldbestoredat4-8C. 2.WASHSOLUTION The20XWashSolutionConcentrateisstableuntiltheexpira¬tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionscanbestoredatroomtemperature(1625C)orat4-8C. 3.ENZYME-ANTIBODYCONJUGATE Undilutedhorseradishperoxidaseanti-CRPconjugateshouldbestoredat4-8Canddilutedimmediatelypriortouse.Theworkingconjugatesolutionisstableforupto8hours. 4.CHROMOGEN-SUBSTRATESOLUTION TheSubstrateSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate. 5.STOPSOLUTION TheStopSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate. 6.ANTI-RABBITCRPELISAMICROPLATE Anti-RabbitCRPcoatedwellsarestableuntiltheexpirationdate,andshouldbestoredat4-8Cinsealedfoilpouchwithdesiccantpack. 7.RABBITCRPCALIBRATOR ThelyophilizedRabbitCRPcalibratorshouldbestoredat4Corfrozenuntilreconstituted.Thereconstitutedcalibratorshouldbealiquotedoutandstoredfrozen(Avoidmultiplefreeze-thawcycles).Theworkingcalibratorsolutionsshouldbepreparedimmediatelypriortouseandarestableforupto8hours. | ||||||||||||||||
Reactivity | Rabbit | ||||||||||||||||
KitSize | 96well | ||||||||||||||||
KitPrinciple | GenWayBiotechCRPELISAKit(Rabbit)(GWB-9BF960)isbasedonstandardsandwichenzyme-linkedimmuno-sorbentassaytechnology.AnantibodyforC-reactiveproteinhasbeenpre-coatedontoa96-wellplate(12x8WellStrips).Standardsortestsamplesareaddedtothewells,incubatedandwashedtoremoveunboundproteins.AnAnti-C-reactiveprotein-HRPconjugateddetectorantibodyisthenadded,incubatedandunboundconjugateiswashedaway.AnenzymaticreactionisproducedthroughtheadditionofTMBsubstratewhichiscatalyzedbyHRPgeneratingabluecolorproductthatchangestoyellowafteraddingacidicstopsolution.Thedensityofyellowcolorationreadbyabsorbanceat450nmisquantitativelyproportionaltotheamountofsampleC-reactiveproteincapturedinthewell. | ||||||||||||||||
KitComponent |
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KitDuration | 25Minutes | ||||||||||||||||
Storage | Refertokitinstructionsforcomponentstorage | ||||||||||||||||
LeadTime | Domestic:within1weekdelivery International:1week | ||||||||||||||||
IntendedUse | ResearchUseOnly | ||||||||||||||||
AdditionalInformation | IntendedUse:TheCRPtestkitisahighlysensitivetwo-siteenzymelinkedimmunoassay(ELISA)formeasuringCRPinRabbitbiologicalsamples. | ||||||||||||||||
:: | Reagents: 1.DILUENTCONCENTRATE(RunningBuffer) Onebottlecontaining50mlofa5Xconcentrateddiluentrunningbuffer. 2.WASHSOLUTIONCONCENTRATE Onebottlecontaining50mlofa20Xconcentratedwashsolution. 3.ENZYME-ANTIBODYCONJUGATE100X Onevialcontaining150ulofaffinitypurifiedanti-RabbitCRPantibodyconjugatedwithhorseradishperoxidaseinastABIlizingbuffer. 4.CHROMOGEN-SUBSTRATESOLUTION Onevialcontaining12mLof3,3’,5,5’-tetramethybenzidine(TMB)andhydrogenperoxideincitricacidbufferatpH3.3. 5.STOPSOLUTION Onevialcontaining12ml0.3Msulfuricacid. WARNING:Avoidcontactwithskin. 6.ANTI-RABBITCRPELISAMICROPLATE Twelveremovableeight(8)wellmicrowellstripsinwellholderframe.Eachwelliscoatedwithaffinitypurifiedanti-RabbitCRP. 7.RABBITCRPCALIBRATOR OnevialcontainingalyophilizedRabbitCRPcalibrator. |