产品资料

GenWay/Hsp90a assay kit/GWB-9C936C/96reactions

Description:

Description:TheHsp90alphaAssayKitisdesignedforidentificationofHsp90alphainhibitorsusingfluorescencepolarization.TheassayisbasedonthecompetitionoffluorescentlylabeledgeldanamycinforbindingtopurifiedrecombinantHsp90alpha.Hsp90alphaisamolecularchaperonewithessentialfunctionsinmaintainingtransformation.InhibitionofHsp90alphafunctionshasbeenshowntoplayaroleintumOrigenesisanddiseaseprogression.TheHsp90alphainhibitorscreeningassaykitcomesinaconvenient96-wellformat,withpurifiedHsp90alphaenzyme,fluorescentlylabeledgeldanamycinandHsp90alphaassaybufferfor96enzymereactions.ThekeytotheHsp90alphaAssayKitisthefluorescentlylabeledgeldanamycin.Usingthiskit,onlyonesimplesteponamicrotiterplateisrequiredforHsp90alphareactions.ThefluorescentlylabeledgeldanamycinisincubatedwithasamplecontainingHsp90alphaenzymetoproduceachangeinfluorescentpolarizationthatcanthenbemeasuredusingafluorescencereader.COMPONENTS:HSP90arecombinantenzyme,FITC-labeledgeldanamycin,5Xassaybuffer,blackmicrotiterplate.APPLICATIONS:GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.STABILITY:1yearwhenstoredaslisted.REFERENCES:1.KimJ,FeltsS,LLaugerL,HeH,HuezoH,RosenN,andChiosisG.JBiomolScreening2004;9(5):375-381.2.HowesR,BarrilX,DymockBW,GrantK,NorthfieldCJ,RobertsonAGS,SurgenorA,WayneJ,WrightL,JamesK,MatthewsT,CheungKM,McDonaldE,WorkmanP,DrysdaleMJ.AnalBiochem2006;350:202-213.

Format:assaykit

ScientificCategory:Misc.HeatShockProteins

AdditionalInformation:

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Name	Hsp90aassaykit
RelatedProductNames	Hsp90aassaykit
Purity	NA
Format	AssayKit
Storage	Mixedstorage
IntendedUse	ResearchUseOnly
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GenWay/Hsp90a assay kit/GWB-9C936C/96reactions

Description:

AdditionalInformation: