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GenWay/IGG Antibodies to Rubella Virus ELISA Quantitation Kit/GWB-9CAA5A/ELISA_Kits

Description:

Species:Human

SampleType:Serum

ForthequantitativedeterminationofIgGAntibodiestoRubellaVirusinserum.RNAVirusInfections,Blood,Infection,PregnancyComplications,PathologicProcesses

ProductName:
IGGAntibodiestoRubellaVirus

SampleType:Serum

#ofSamples:1X96Assays

IntendedUse:TheGenWayBiotechRubellaIgGELISAisintendedforuseinevaluatingSEROlogicstatustotherubellavirusinfection.ItisalsousedtoevaluatepairedseraforthepresenceofasignificantincreaseinspecificRubellaIgGasindicationofarecentorcurrentrubellavirusinfection.

Introduction:Rubellaisaherpesvirus.Generallyrubellaisconsideredamildadolescentdisease.However,amaternalinfectioncanbetransmittedthroughtheplacentatothefetus,causingcongenitalrubella.Congenitalrubellamayresultinchroniccardiacdisease,growthretardationhepatosplenomegaly,malformationsandothersevereanomalies.Childrenbornasymptomaticmaydeveloptheseabnormalitieslaterinlife.Toreducetheriskofsuchseverecomplications,accurateserologicalmethodsmustbeperformedtodeterminetheserologicstatusofchildbearing-agedwomen.Thepresenceofrubella-specificIgGinthebloodstreamattestsimmunitytorubella.Awomantestedtobenon-immunecanbeeducatedoninfectionanddifferentiaterubellafromotherexanthematousdiseases.Expectingwomenwithcurrentrubellainfectionshouldbecounseledontheconsequencesofcongenitalinfection.

Principleofthetest:PurifiedRubellaantigeniscoatedonthesurfaceofmicrowells.Dilutedserumisaddedtothewells,andtheRubellaIgG-specificantibody,ifpresent,bindstotheantigenduringincubation.Afterwashingthewellstoremoveunboundsample,antibodytohumanIgGconjugatedwithhorserADIshperoxidase(HRP)isaddedandincubatedat37Cfor30minutes.Unboundconjugateisremovedbyasubsequentwashingstep.AsolutionofTMBReagentisthenaddedtothemicrowells.Theenzymeconjugatecatalyticreactionisstoppedataspecifictime.TheintensityofthecolorgeneratedisproportionaltotheamountofIgG-specificantibodyinthesample.Theresultsarereadbyamicrowellreadercomparedinaparallelmannerwithcalibratorsandcontrols.

LimitationoftheTest:1.ReliableandreproducIBLeresultswillbeobtainedwhentheassayprocedureiscarriedoutwithacompleteunderstandingofthepackageinsertinstructionsandwithadherencetogoodlaboratorypractice.
2.Thewashprocedureiscritical.Insufficientwashingwillresultinpoorprecisionandfalselyelevatedabsorbancereadings.
3.Serumsamplesdemonstratinggrosslipemia,grosshemolysis,orturbidityshouldnotbeusedwiththistest.

Precision:AstudywasperformedtodocumenttypicalassayprecisionwiththeGenWayBiotechRubellaIgGEIAproduct.Themean,SD,and%CVwerecalculatedforIntra-andInter-Assayinconcentrationvalue(IU/mL).

ProductName:IGGAntibodiestoRubellaVirus

SubcellularLocation:
Secreted.

Disease:ChromosomalaberrationsinvolvingIGHG1maybeacauseofmultiplemyeloma[MIM:254500].Translocationt(11;14)(q13;q32)withCCND1;translocationt(4;14)(p16.3;q32.3)withFGFR3;translocationt(6;14)(p25;q32)withIRF4.

Miscellaneous:NiehastheG1M(17)allotypicMarker,97-K,andtheG1M(1)markers,239-Dand241-L.KOLandEUsequenceshavetheG1M(3)markerandtheG1M(non-1)markers.

Miscellaneous:Niealsodiffersintheamidationstatesof35,116,198,269and272.

Miscellaneous:EUalsodiffersintheamidationstatesofresidues155,166,177,195,198,269,and272andintheorderofresidues268-272.

Miscellaneous:KOLalsodiffersintheamidationstatesofresidues198,267and272.

AdditionalInformation:

NameIGGAntibodiestoRubellaVirusELISAQuantitationKit
RelatedProductNamesIgGAntibodiestoRubellaVirusELISAKit;N/AIgGAntibodiestoRubellaVirusIGHG1
MolecularWeight36106
SwissProtNumberP01857
IntendedUseResearchUseOnly