Name | BovineHaptoglobinELISAKit | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
RelatedProductNames | HP2-ALPHA;BovineHaptoglobinELISAKit;N/ABovineHaptoglobinHP | ||||||||||||||||
Geneid | 280692 | ||||||||||||||||
AliasSymbols | Haptoglobin,HP,Zonulin | ||||||||||||||||
ProteinName | Haptoglobin | ||||||||||||||||
DescriptionofTarget | Asaresultofhemolysis,hemoglobinisfoundtoaccumulateinthekidneyandissecretedintheurine.Haptoglobincaptures,andcombineswithfreeplasmahemoglobintoallowhepaticrecyclingofhemeironandtopreventkidneydamage.Haptoglobinalsoa | ||||||||||||||||
SwissprotID | Q2TBU0 | ||||||||||||||||
SpeciesReactivity | Cow | ||||||||||||||||
AssayInfo | AssayMethodology:QuantitativeSandwichELISA | ||||||||||||||||
Sensitivity | 6.258ng/ml | ||||||||||||||||
KitRange | 15.6ng/ml-1,000ng/ml | ||||||||||||||||
Homology | Cow | ||||||||||||||||
NCBIAccNumber | NP_001119574.1 | ||||||||||||||||
StandardRange | ReferToManual | ||||||||||||||||
SampleType | Serum,plasmaandotherBIOLOGicalfluids | ||||||||||||||||
ProductFormat | InSolution | ||||||||||||||||
Specimen/Volumes | ReferToManual | ||||||||||||||||
SwissProtNumber | P00738 | ||||||||||||||||
Applications | ELISA | ||||||||||||||||
ReconstitutionandStorage | 1.DILUENT The5XDiluentConcentrateisstableuntiltheexpira-tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionsshouldbestoredat4-8C. 2.WASHSOLUTION The20XWashSolutionConcentrateisstableuntiltheexpira¬tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionscanbestoredatroomtemperature(1625C)orat4-8C. 3.ENZYME-ANTIBODYCONJUGATE Undilutedhorseradishperoxidaseanti-Hpconjugateshouldbestoredat4-8Canddilutedimmediatelypriortouse.Theworkingconjugatesolutionisstableforupto8hours. 4.CHROMOGEN-SUBSTRATESOLUTION TheSubstrateSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate. 5.STOPSOLUTION TheStopSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate. 6.ANTI-BOVINEHaptoglobinELISAMICROPLATE Anti-BovineHpcoatedwellsarestableuntiltheexpirationdate,andshouldbestoredat4-8Cinsealedfoilpouchwithdesiccantpack. 7.BOVINEHaptoglobinCALIBRATOR ThelyophilizedBovineHaptoglobincalibratorshouldbestoredat4Corfrozenuntilreconstituted.Thereconstitutedcalibratorshouldbealiquotedoutandstoredfrozen(Avoidmultiplefreeze-thawcycles).Theworkingstandardsolutionsshouldbepreparedimmediatelypriortouseandarestableforupto8hours. | ||||||||||||||||
Reactivity | Bovine | ||||||||||||||||
KitSize | 96well | ||||||||||||||||
KitPrinciple | GenWayBiotechHaptoglobinELISAKit(Bovine)(GWB-A43096)isbasedonstandardsandwichenzyme-linkedimmuno-sorbentassaytechnology.AnantibodyforHaptoglobinhasbeenpre-coatedontoa96-wellplate(12x8WellStrips).Standardsortestsamplesareaddedtothewells,incubatedandwashedtoremoveunboundproteins.AnAnti-Haptoglobin-HRPconjugateddetectorantibodyisthenadded,incubatedandunboundconjugateiswashedaway.AnenzymaticreactionisproducedthroughtheadditionofTMBsubstratewhichiscatalyzedbyHRPgeneratingabluecolorproductthatchangestoyellowafteraddingacidicstopsolution.Thedensityofyellowcolorationreadbyabsorbanceat450nmisquantitativelyproportionaltotheamountofsampleHaptoglobincapturedinthewell. | ||||||||||||||||
KitComponent |
| ||||||||||||||||
KitDuration | 40Minutes | ||||||||||||||||
Storage | Refertokitinstructionsforcomponentstorage | ||||||||||||||||
LeadTime | Domestic:within1weekdelivery International:1week | ||||||||||||||||
IntendedUse | ResearchUseOnly | ||||||||||||||||
AdditionalInformation | IntendedUse:TheHaptoglobintestkitsareahighlysensitivetwo-siteenzymelinkedimmunoassay(ELISA)formeasuringHaptoglobininbiologicalsamplesofcattle. |