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GenWay/Bovine Haptoglobin ELISA Kit/GWB-A43096/96 well

Description:

ShortDescription:InthisassaytheHAPTOGLOBINpresentinserumsamplereactswiththeanti-HAPTOGLOBINantibodieswhichhavebeenadsorbedtothesurfaceofpolystyrenemicrotitrewells.Aftertheremovalofunboundserumproteinsbywashing,anti-HpantibodiesconjugatedwithhorserADIshperoxidase(HRP),areadded.Theseenzyme-labeledantibodiesformcomplexeswiththepreviouslyboundserumHp.Followinganotherwashingstep,theenzymeboundtotheimmunosorbentisassayedbytheadditionofachromogenicsubstrate,3,3’,5,5’-tetramethylbenzidine(TMB).ThequantityofboundenzymevariesdirectlywiththeconcentrationofHpinthesampletested;thus,theabsorbance,at450nm,isameasureoftheconcentrationofHpinthetestsample.ThequantityofHpinthetestsamplecanbeinterpolatedfromthestandardcurveconstructedfromthestandards,andcorrectedforserumdilution.

AdditionalInformation:

NameBovineHaptoglobinELISAKit
RelatedProductNamesHP2-ALPHA;BovineHaptoglobinELISAKit;N/ABovineHaptoglobinHP
Geneid280692
AliasSymbolsHaptoglobin,HP,Zonulin
ProteinNameHaptoglobin
DescriptionofTargetAsaresultofhemolysis,hemoglobinisfoundtoaccumulateinthekidneyandissecretedintheurine.Haptoglobincaptures,andcombineswithfreeplasmahemoglobintoallowhepaticrecyclingofhemeironandtopreventkidneydamage.Haptoglobinalsoa
SwissprotIDQ2TBU0
SpeciesReactivityCow
AssayInfoAssayMethodology:QuantitativeSandwichELISA
Sensitivity6.258ng/ml
KitRange15.6ng/ml-1,000ng/ml
HomologyCow
NCBIAccNumberNP_001119574.1
StandardRangeReferToManual
SampleTypeSerum,plasmaandotherBIOLOGicalfluids
ProductFormatInSolution
Specimen/VolumesReferToManual
SwissProtNumberP00738
ApplicationsELISA
ReconstitutionandStorage1.DILUENT
The5XDiluentConcentrateisstableuntiltheexpira-tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionsshouldbestoredat4-8C.
2.WASHSOLUTION
The20XWashSolutionConcentrateisstableuntiltheexpira¬tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionscanbestoredatroomtemperature(1625C)orat4-8C.
3.ENZYME-ANTIBODYCONJUGATE
Undilutedhorseradishperoxidaseanti-Hpconjugateshouldbestoredat4-8Canddilutedimmediatelypriortouse.Theworkingconjugatesolutionisstableforupto8hours.
4.CHROMOGEN-SUBSTRATESOLUTION
TheSubstrateSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate.
5.STOPSOLUTION
TheStopSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate.
6.ANTI-BOVINEHaptoglobinELISAMICROPLATE
Anti-BovineHpcoatedwellsarestableuntiltheexpirationdate,andshouldbestoredat4-8Cinsealedfoilpouchwithdesiccantpack.
7.BOVINEHaptoglobinCALIBRATOR
ThelyophilizedBovineHaptoglobincalibratorshouldbestoredat4Corfrozenuntilreconstituted.Thereconstitutedcalibratorshouldbealiquotedoutandstoredfrozen(Avoidmultiplefreeze-thawcycles).Theworkingstandardsolutionsshouldbepreparedimmediatelypriortouseandarestableforupto8hours.
ReactivityBovine
KitSize96well
KitPrincipleGenWayBiotechHaptoglobinELISAKit(Bovine)(GWB-A43096)isbasedonstandardsandwichenzyme-linkedimmuno-sorbentassaytechnology.AnantibodyforHaptoglobinhasbeenpre-coatedontoa96-wellplate(12x8WellStrips).Standardsortestsamplesareaddedtothewells,incubatedandwashedtoremoveunboundproteins.AnAnti-Haptoglobin-HRPconjugateddetectorantibodyisthenadded,incubatedandunboundconjugateiswashedaway.AnenzymaticreactionisproducedthroughtheadditionofTMBsubstratewhichiscatalyzedbyHRPgeneratingabluecolorproductthatchangestoyellowafteraddingacidicstopsolution.Thedensityofyellowcolorationreadbyabsorbanceat450nmisquantitativelyproportionaltotheamountofsampleHaptoglobincapturedinthewell.
KitComponent
ComponentAmount
Anti-BovineHaptoglobinMicroplate96Wells(12x8WellStrips)
20XDiluentConcentrate50mL
20XWashSolution50mL
100XEnzyme-AntibodyConjugate150uL
Chromogen-SubstrateSolution12mL
StopSolution12mL
BovineHaptoglobinCalibrator1
KitDuration40Minutes
StorageRefertokitinstructionsforcomponentstorage
LeadTimeDomestic:within1weekdelivery International:1week
IntendedUseResearchUseOnly
AdditionalInformationIntendedUse:TheHaptoglobintestkitsareahighlysensitivetwo-siteenzymelinkedimmunoassay(ELISA)formeasuringHaptoglobininbiologicalsamplesofcattle.