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GenWay/Rat CRP (C-Reactive Protein) ELISA Kit/GWB-A7EDCA/96 well188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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GenWay/Rat CRP (C-Reactive Protein) ELISA Kit/GWB-A7EDCA/96 well

Description:

ShortDescription:InthisassaytheCRPpresentinserumsamplereactswiththeanti-CRPantibodieswhichhavebeenadsorbedtothesurfaceofpolystyrenemicrotitrewells.Aftertheremovalofunboundserumproteinsbywashing,anti-CRPantibodiesconjugatedwithhorserADIshperoxidase(HRP),areadded.Theseenzyme-labeledantibodiesformcomplexeswiththepreviouslyboundserumCRP.Followinganotherwashingstep,theenzymeboundtotheimmunosorbentisassayedbytheadditionofachromogenicsubstrate,3,3’,5,5’-tetramethylbenzidine(TMB).ThequantityofboundenzymevariesdirectlywiththeconcentrationofCRPinthesampletested;thus,theabsorbance,at450nm,isameasureoftheconcentrationofCRPinthetestsample.ThequantityofCRPinthetestsamplecanbeinterpolatedfromthestandardcurveconstructedfromthestandards,andcorrectedforserumdilution.

AdditionalInformation:

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NameRatCRP(C-ReactiveProtein)ELISAKit
RelatedProductNamesRatCRP(C-ReactiveProtein)ELISAKit;N/APTX1RatCRP(C-ReactiveProtein)CRP
GeneSymbolCRP
GeneFullnameC-reactiveprotein
Geneid25419
AliasSymbolsCRP,PTX1
ProteinNameC-reactiveprotein
DescriptionofTargetDisplaysseveralfunctionsassociatedwithhostdefense:itpromotesagglutination,bacterialcapsularswelling,phagocytosisandcomplementfixationthroughitscalcium-dependentbindingtophosphorylcholine.CaninteractwithDNAandhistonesandmaysc
SwissprotIDP48199
SpeciesReactivityRat
AssayInfoAssayMethodology:QuantitativeSandwichELISA
Sensitivity1.266ng/ml
KitRange6.25ng/ml-200ng/ml
HomologyRat
NCBIAccNumberNP_000558.2
StandardRangeReferToManual
SampleTypeSerum,plasmaandotherBIOLOGicalfluids
ProductFormatInSolution
Specimen/VolumesReferToManual
SwissProtNumberP02741
ApplicationsELISA
ReconstitutionandStorage1.DILUENT
The5XDiluentConcentrateisstableuntiltheexpira-tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionsshouldbestoredat4-8C.
2.WASHSOLUTION
The20XWashSolutionConcentrateisstableuntiltheexpira¬tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionscanbestoredatroomtemperature(1625C)orat4-8C.
3.ENZYME-ANTIBODYCONJUGATE
Undilutedhorseradishperoxidaseanti-CRPconjugateshouldbestoredat4-8Canddilutedimmediatelypriortouse.Theworkingconjugatesolutionisstableforupto8hours.
4.CHROMOGEN-SUBSTRATESOLUTION
TheSubstrateSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate.
5.STOPSOLUTION
TheStopSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate.
6.ANTI-RATCRPELISAMICROPLATE
Anti-RatCRPcoatedwellsarestableuntiltheexpirationdate,andshouldbestoredat4-8Cinsealedfoilpouchwithdesiccantpack.
7.RATCRPSTANDARDS
ThelyophilizedRatCRPcalibratorshouldbestoredat4Corfrozenuntilreconstituted.Thereconstitutedcalibratorshouldbealiquotedoutandstoredfrozen(Avoidmultiplefreeze-thawcycles).Theworkingstandardsolutionsshouldbepreparedimmediatelypriortouseandarestableforupto8hours.
ReactivityRat
KitSize96well
KitPrincipleGenWayBiotechCRPELISAKit(Rat)(GWB-A7EDCA)isbasedonstandardsandwichenzyme-linkedimmuno-sorbentassaytechnology.AnantibodyforC-reactiveproteinhasbeenpre-coatedontoa96-wellplate(12x8WellStrips).Standardsortestsamplesareaddedtothewells,incubatedandwashedtoremoveunboundproteins.AnAnti-C-reactiveprotein-HRPconjugateddetectorantibodyisthenadded,incubatedandunboundconjugateiswashedaway.AnenzymaticreactionisproducedthroughtheadditionofTMBsubstratewhichiscatalyzedbyHRPgeneratingabluecolorproductthatchangestoyellowafteraddingacidicstopsolution.Thedensityofyellowcolorationreadbyabsorbanceat450nmisquantitativelyproportionaltotheamountofsampleC-reactiveproteincapturedinthewell.
KitComponent
ComponentAmount
Anti-RatCRPMicroplate96Wells(12x8WellStrips)
20XDiluentConcentrate50mL
20XWashSolution50mL
100XEnzyme-AntibodyConjugate150uL
Chromogen-SubstrateSolution12mL
StopSolution12mL
RatCRPCalibrator1
KitDuration25Minutes
StorageRefertokitinstructionsforcomponentstorage
LeadTimeDomestic:within1weekdelivery International:1week
IntendedUseResearchUseOnly
AdditionalInformationIntendedUse:TheCRPtestkitsareahighlysensitivetwo-siteenzymelinkedimmunoassay(ELISA)formeasuringCRPinbiologicalsamplesofrats.
::Reagents:
1.DILUENTCONCENTRATE(RunningBuffer)
Onebottlecontaining50mlofa5Xconcentrateddiluentrunningbuffer.
2.WASHSOLUTIONCONCENTRATE
Onebottlecontaining50mlofa20Xconcentratedwashsolution.
3.ENZYME-ANTIBODYCONJUGATE100X
Onevialcontaining150ulofaffinitypurifiedanti-RatCRPantibodyconjugatedwithhorseradishperoxidaseinastABIlizingbuffer.
4.CHROMOGEN-SUBSTRATESOLUTION
Onevialcontaining12mLof3,3’,5,5’-tetramethybenzidine(TMB)andhydrogenperoxideincitricacidbufferatpH3.3.
5.STOPSOLUTION
Onevialcontaining12ml0.3Msulfuricacid.
WARNING:Avoidcontactwithskin.
6.ANTI-RATCRPELISAMICROPLATE
Twelveremovableeight(8)wellmicrowellstripsinwellholderframe.Eachwelliscoatedwithaffinitypurifiedanti-RatCRP.
7.RATCRPCALIBRATOR
OnevialcontainingalyophilizedRatCRPcalibrator.