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GenWay/Rat C3 (Complement Factor 3) ELISA Kit/GWB-A8B8AF/96 well188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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GenWay/Rat C3 (Complement Factor 3) ELISA Kit/GWB-A8B8AF/96 well

Description:

ShortDescription:InthisassaytheC3presentinserumsamplereactswiththeanti-C3.antibodieswhichhavebeenadsorbedtothesurfaceofpolystyrenemicrotitrewells.Aftertheremovalofunboundserumproteinsbywashing,anti-C3.antibodiesconjugatedwithhorserADIshperoxidase(HRP),areadded.Theseenzyme-labeledantibodiesformcomplexeswiththepreviouslyboundserumC3.Followinganotherwashingstep,theenzymeboundtotheimmunosorbentisassayedbytheadditionofachromogenicsubstrate,3,3’,5,5’-tetramethylbenzidine(TMB).ThequantityofboundenzymevariesdirectlywiththeconcentrationofC3inthesampletested;thus,theabsorbance,at450nm,isameasureoftheconcentrationofC3inthetestsample.ThequantityofC3inthetestsamplecanbeinterpolatedfromthestandardcurveconstructedfromthestandards,andcorrectedforserumdilution.

AdditionalInformation:

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NameRatC3(ComplementFactor3)ELISAKit
RelatedProductNamesRatC3(ComplementFactor3)ELISAKit;C3andPZP-likealpha-2-macroglobulindomain-containingprotein1CPAMD1RatC3(ComplementFactor3)C3
GeneSymbolC3
GeneFullnamecomplementC3
Geneid24232
AliasSymbolsComplementC3,C3,CPAMD1
ProteinNameComplementC3
DescriptionofTargetC3playsacentralroleintheactivationofthecomplementsystem.ItsprocessingbyC3convertaseisthecentralreactioninbothclassicalandalternativecomplementpathways.AfteractivationC3bcanbindcovalently,viaitsreactivethioester,tocel
SwissprotIDP01026
SpeciesReactivityRat
AssayInfoAssayMethodology:QuantitativeSandwichELISA
Sensitivity4.326ng/ml
KitRange12.5ng/ml-800ng/ml
HomologyRat
NCBIAccNumberNP_000055.2
StandardRangeReferToManual
SampleTypeSerum,plasmaandotherBIOLOGicalfluids
ProductFormatInSolution
Specimen/VolumesReferToManual
SwissProtNumberP01024
ApplicationsELISA
ReconstitutionandStorage1.DILUENT
The5XDiluentConcentrateisstableuntiltheexpira-tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionsshouldbestoredat4-8C.
2.WASHSOLUTION
The20XWashSolutionConcentrateisstableuntiltheexpira¬tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionscanbestoredatroomtemperature(1625C)orat4-8C.
3.ENZYME-ANTIBODYCONJUGATE
Undilutedhorseradishperoxidaseanti-C3conjugateshouldbestoredat4-8Canddilutedimmediatelypriortouse.Theworkingconjugatesolutionisstableforupto8hours.
4.CHROMOGEN-SUBSTRATESOLUTION
TheSubstrateSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate.
5.STOPSOLUTION
TheStopSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate.
6.ANTI-RATC3ELISAMICROPLATE
Anti-RatC3coatedwellsarestableuntiltheexpirationdate,andshouldbestoredat4-8Cinsealedfoilpouchwithdesiccantpack.
7.RATC3CALIBRATOR
ThelyophilizedRatC3calibratorshouldbestoredat4Corfrozenuntilreconstituted.Thereconstitutedcalibratorshouldbealiquotedoutandstoredfrozen(Avoidmultiplefreeze-thawcycles).Theworkingstandardsolutionsshouldbepreparedimmediatelypriortouseandarestableforupto8hours.
ReactivityRat
KitSize96well
KitPrincipleGenWayBiotechC3ELISAKit(Rat)(GWB-A8B8AF)isbasedonstandardsandwichenzyme-linkedimmuno-sorbentassaytechnology.AnantibodyforComplementC3hasbeenpre-coatedontoa96-wellplate(12x8WellStrips).Standardsortestsamplesareaddedtothewells,incubatedandwashedtoremoveunboundproteins.AnAnti-ComplementC3-HRPconjugateddetectorantibodyisthenadded,incubatedandunboundconjugateiswashedaway.AnenzymaticreactionisproducedthroughtheadditionofTMBsubstratewhichiscatalyzedbyHRPgeneratingabluecolorproductthatchangestoyellowafteraddingacidicstopsolution.Thedensityofyellowcolorationreadbyabsorbanceat450nmisquantitativelyproportionaltotheamountofsampleComplementC3capturedinthewell.
KitComponent
ComponentAmount
Anti-RatC3Microplate96Wells(12x8WellStrips)
20XDiluentConcentrate50mL
20XWashSolution50mL
100XEnzyme-AntibodyConjugate150uL
Chromogen-SubstrateSolution12mL
StopSolution12mL
RatC3Calibrator1
KitDuration50Minutes
StorageRefertokitinstructionsforcomponentstorage
LeadTimeDomestic:within1weekdelivery International:1week
IntendedUseResearchUseOnly
AdditionalInformationIntendedUse:TheComplementfactor3(C3)testkitsarehighlysensitivetwo-siteenzymelinkedimmunoassay(ELISA)formeasuringC3inbiologicalsamplesofrats.
::Reagents:
1.DILUENTCONCENTRATE(RunningBuffer)
Onebottlecontaining50mlofa5Xconcentrateddiluentrunningbuffer.
2.WASHSOLUTIONCONCENTRATE
Onebottlecontaining50mlofa20Xconcentratedwashsolution.
3.ENZYME-ANTIBODYCONJUGATE100X
Onevialcontaining150ulofaffinitypurifiedanti-RatC3antibodyconjugatedwithhorseradishperoxidaseinastABIlizingbuffer.
4.CHROMOGEN-SUBSTRATESOLUTION
Onevialcontaining12mLof3,3’,5,5’-tetramethybenzidine(TMB)andhydrogenperoxideincitricacidbufferatpH3.3.
5.STOPSOLUTION
Onevialcontaining12ml0.3Msulfuricacid.
WARNING:Avoidcontactwithskin.
6.ANTI-RATC3ELISAMICROPLATE
Twelveremovableeight(8)wellmicrowellstripsinwellholderframe.Eachwelliscoatedwithaffinitypurifiedanti-RatC3.
7.RATC3CALIBRATOR
OnevialcontainingalyophilizedRatC3calibrator.