GenWay/Cortisol ELISA (Sheep)/GWB-AEB745/ELISA_Kits

Description:

INTENDED USE  The GenWay, Inc. Cortisol ELISA kit is used for the quantitative measurement of Cortisol in sheep serum or plasma.

SUMMARY AND EXPLANATION  Cortisol (hydrocortisone, compound F) is the most potent glucocorticoid synthesized from cholesterol. Cortisol is found the blood either as free Cortisol, or bound to corticosteroid-binding globulin (CBG). Cortisol production has an ACTHdependent circadian rhythm with peak levels in the early morning and a nadir at night. The factors controlling this circadian rhythm are not completely defined. Serum levels are highest in the early morning and decrease throughout the day. In the metabolic aspect, Cortisol promotes gluconeogenesis, liver glycogen deposition, and the reduction of glucose utilization. Immunologically, Cortisol functions as an important anti inflammatory, and plays a role in hypersensitivity, immunosuppression, and disease resistance.  This kit can detect low level of cortisol in sheep serum or plasma (less than 0.1 ng/ml). 

PRINCIPLE OF THE TEST The CBI Cortisol is a solid phase competitive ELISA. The samples and Cortisol enzyme conjugate are added to the wells coated with anti-Cortisol monoclonal antibody. Cortisol in the patient’s sample competes with a Cortisol enzyme conjugate for binding sites. Unbound Cortisol and Cortisol enzyme conjugate is washed off by washing buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of Cortisol in the samples. A standard curve is prepared relating color intensity to theconcentration of the Cortisol. 

SENSITIVITY This assay sensitivity was found to be 0.1ng/ml. The sensitivity was determined by calculating the mean plus 2SD of the standard zero point tested 20 times in the same run. 

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