1. Intended Use
The GenWay Adenovirus IgG Antibody ELISA Test Kit has been designed for the the detection and the quantitative determination of specific IgG antibodies against Adenovirus in serum and plasma. Further applications in other body fluids are possible and can be requested from the Technical Service of GenWay. This assay is intended for research use only.
2. General Information
The adenovirus is an ubiquitous pathogen of humans and animals. Adenoviruses are characterized by location inside the cell nucleus, common complement-fixing antigens and marked stability to environmental effects. Adenoviruses are endemic in all populations throughout the year. The infection is spread both through the aerial-droplet route and the routes characteristic for intestinal infections. The incubation period is between five and seven days. Adenoviruses mainly infest respiratory and intestinal mucosa, but also the cornea. They are accumulated in the epithelial cells and regional lymph nodes. Adenoviruses cause the widest variety of illnesses of the known respiratory viruses. The adenovirus infection is the most frequently caused viral disease of the respiratory tract among preschool children (types 1 - 5 and 7). Acute diseases of the upper respiratory tract occur predominantly. Pneumonia is the most severe form of adenoviral infection occurring mostly in infants below the age of one. Adenoviruses also cause outbreaks of swimmingpool-associated pharyngoconjunctival fever in the summer and epidemics of kerato-conjunctivitis of both children and adults. The intestinal form of adenoviral infection occurs mostly in children below the age of one. An acute adenoviral infection can be detected by virus isolation and/or serology. The serologic tests are particularly important because they document actual infection in the individual and can be applied to large-scale epidemiologic investigations. The CF and ELISA tests measure predominantly the antibodies directed against the group-specific determinants on the hexon component. The recommended tests for measuring type-specific antibodies are hemagglutinin inhibition and serum neutralization. The type-specific antigenic determinants of adenoviruses are located at the fibers on the capsid. Because of the ubiquity of the adenoviruses and numerous cross-reactions between related serotypes, seroconversion involving a fourfold or greater rise in antibody infection is necessary to document infection. IgG is the predominant antibody class measured in the serologic tests.
3. Principle of the Test
The GenWay Adenovirus IgG antibody test kit is based on the principle of the enzyme immunoassay (EIA). Adenovirus antigen is bound on the surface of the microtiter strips. Diluted sample serum or ready-to-use standards are pipetted into the wells of the microtiter plate. A binding between the IgG antibodies of the serum and the immobilized Adenovirus antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use anti-human-IgG peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of the IgG antibodies is directly proportional to the intensity of the color.
Name | Adenovirus IgG ELISA |
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Related Product Names | Adenovirus IgG ELISA |
Applications | Enzyme immunoassay based on microtiter plate for the detection and quantitative determination of human IgG antibodies against Adenovirus in serum and plasma |
Intended Use | Research Use Only |
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