产品资料

GenWay/Human Myoglobin ELISA Kit/GWB-AF81CB/96 well

Description:

Short Description: Myoglobin is a single-chain globular protein with a molecular weight of 16,700 daltons. It contains a heme prosthetic group in the center and is the primary oxygen-carrying pigment of muscle tissues. Myoglobin is released from damaged muscle tissue which is filtered by the kidney. therefore is a sensitive marker for muscle injury.

Additional Information:

Name

Human Myoglobin ELISA Kit

Related Product Names

Human Myoglobin ELISA Kit; N/A Human MyoglobinMB

Gene Symbol

Gene Fullname

myoglobin

Gene id

4151

Alias Symbols

MB, Myoglobin

Protein Name

Myoglobin

Description of Target

Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.

Swissprot ID

P02144

Species Reactivity

Human

Assay Info

Assay Methodology: Quantitative Sandwich ELISA

Sensitivity

0.540 ng/ml

Kit Range

2.5 ng/ml - 80 ng/ml

Homology

Human

NCBI Acc Number

NP_005359.1

Standard Range

Refer To Manual

Sample Type

Serum, plasma and other biological fluids

Product Format

In Solution

Specimen/Volumes

Refer To Manual

Swiss Prot Number

P02144

Applications

ELISA

Reconstitution and Storage

1. DILUENT
The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.
2. WASH SOLUTION
The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.
3. ENZYME-ANTIBODY CONJUGATE
Undiluted horseradish peroxidase anti-Myoglobin conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.
4. CHROMOGEN-SUBSTRATE SOLUTION
The Substrate Solution should be stored at 4-8C and is stable until the expiration date.
5. STOP SOLUTION
The Stop Solution should be stored at 4-8C and is stable until the expiration date.
6. ANTI-HUMAN MYOGLOBIN ELISA MICRO PLATE
Anti-Human Myoglobin coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.
7. HUMAN MYOGLOBIN CALIBRATOR
The lyophilized Human Myoglobin calibrator should be stored at 4C or frozen until reconstituted. The reconstituted calibrator should be aliquoted out and stored frozen (Avoid multiple freeze-thaw cycles). The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours.

Reactivity

Human

Kit Size

96 well

Kit Principle

Genway Biotech Myoglobin ELISA Kit (Human) (GWB-AF81CB) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Myoglobin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Myoglobin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Myoglobin captured in the well.

Kit Component

Component	Amount
Anti-Human Myoglobin Microplate	96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate	50 mL
20X Wash Solution	50 mL
100X Enzyme-Antibody Conjugate	150 uL
Chromogen-Substrate Solution	12 mL
Stop Solution	12 mL
Human Myoglobin Calibrator	1

Kit Duration

100 Minutes

Storage

Refer to kit instructions for component storage

Lead Time

Domestic: within 1 week delivery International: 1 week

Intended Use

Research Use Only

Additional Information

Intended Use: The Myoglobin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Myoglobin in biological samples of Human.

Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Human Myoglobin antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-HUMAN MYOGLOBIN ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human Myoglobin.
7. HUMAN MYOGLOBIN CALIBRATOR
One vial containing a lyophilized Human Myoglobin calibrator.

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GenWay/Human Myoglobin ELISA Kit/GWB-AF81CB/96 well

Description:

Additional Information: