Name | Mouse IgE ELISA Kit | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Related Product Names | Mouse IgE ELISA Kit; N/A Mouse IgEIGHE | ||||||||||||||||
Alias Symbols | IGHE, Ig epsilon chain C region, IgE | ||||||||||||||||
Protein Name | Ig epsilon chain C region | ||||||||||||||||
Swissprot ID | P06336 | ||||||||||||||||
Species Reactivity | Mouse | ||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||
Sensitivity | 1.911 ng/ml | ||||||||||||||||
Kit Range | 6.25 ng/ml - 400 ng/ml | ||||||||||||||||
Homology | Mouse | ||||||||||||||||
Standard Range | Refer To Manual | ||||||||||||||||
Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||
Product Format | In Solution | ||||||||||||||||
Specimen/Volumes | Refer To Manual | ||||||||||||||||
Swiss Prot Number | P01854 | ||||||||||||||||
Applications | ELISA | ||||||||||||||||
Reconstitution and Storage | 1. DILUENT The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C. 2. WASH SOLUTION The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C. 3. ENZYME-ANTIBODY CONJUGATE Undiluted horseradish peroxidase anti-IgE conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours. 4. CHROMOGEN-SUBSTRATE SOLUTION The Substrate Solution should be stored at 4-8C and is stable until the expiration date. 5. STOP SOLUTION The Stop Solution should be stored at 4-8C and is stable until the expiration date. 6. ANTI-MOUSE IgE ELISA MICRO PLATE Anti-Mouse IgE coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack. 7. MOUSE IgE CALIBRATOR Long Term Storage: Upon receipt, aliquot the calibrator and store them frozen. They will be stable until expiration date. Short Term Storage: the calibrator is stable for up to 14 days at 4C. The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours. |
||||||||||||||||
Reactivity | Mouse | ||||||||||||||||
Kit Size | 96 well | ||||||||||||||||
Kit Principle | Genway Biotech IgE ELISA Kit (Mouse) (GWB-B2B047) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Ig epsilon chain C region has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Ig epsilon chain C region - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ig epsilon chain C region captured in the well. | ||||||||||||||||
Kit Component |
|
||||||||||||||||
Kit Duration | 70 Minutes | ||||||||||||||||
Storage | Refer to kit instructions for component storage | ||||||||||||||||
Lead Time | Domestic: within 1 week delivery International: 1 week | ||||||||||||||||
Intended Use | Research Use Only | ||||||||||||||||
Additional Information | Intended Use: The IgE test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgE in biological samples of mice. | ||||||||||||||||
:: | Reagents: 1. DILUENT CONCENTRATE (Running Buffer) One bottle containing 50 ml of a 5X concentrated diluent running buffer. 2. WASH SOLUTION CONCENTRATE One bottle containing 50 ml of a 20X concentrated wash solution. 3. ENZYME-ANTIBODY CONJUGATE 100X One vial containing 150 ul of affinity purified anti-Mouse IgE antibody conjugated with horseradish peroxidase in a stabilizing buffer. 4. CHROMOGEN-SUBSTRATE SOLUTION One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3. 5. STOP SOLUTION One vial containing 12 ml 0.3 M sulfuric acid. WARNING: Avoid contact with skin. 6. ANTI-MOUSE IgE ELISA MICRO PLATE Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Mouse IgE. 7. MOUSE IgE CALIBRATOR One vial containing a Mouse IgE calibrator. |
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn