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| Catalognumber | HM1021 |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 100µg |
| Formulation | 1ml(100µg/ml)0.2µmfilteredantibodysolutioninPBS,containing0.1%bovineserumalbumin. |
| Application | Flowcytometry,Functionalstudies |
| ApplicationNotes | FC:AntibodyV1qstainstheextracellulardomainofmouseTNF-;.TheCHOcellswerefixedinPBAcontaining0.2%formaldehydebeforestaining.AspositivecontrolmTNF-;transfectedcellswereused.(Ref.2) FS:AntibodyV1qfunctionsasaneutralizingantibody.TheantibodywasfunctionallytestedbyneutralizationofthecytopathiceffectofcytotoxinintheL929TNFbioassay.TheBIOLOGicalactivityoftheantibodycanbedefinedastheconcentrationofV1qrequiredtoneutralize100U/mlofTNF/cytotoxin.(Ref.1) |
| Use | Forflowcytometry,dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.Forfunctionalstudies,invitrodilutionshavetobeoptimizedinuser’sexperimentalsetting. |
| Immunogen | CytotoxinpurifiedfromconAinducedTcellclone29 |
| Species | Mouse |
| Alias | TNF,TNF-SF2,DIF,cachectin,TNF-α,tumornecrosisfactorligandsuperfamilymember2 |
| PositiveControl | Monocytes/macrophages |
| References | 1.-Echtenacher,Betal;Requirementofendogenoustumornecrosisfactor/cachectinforrecoveryfromexperimentalperitonitis.JImmunol1990,145:3762 2.-Gerspach,Jetal;Detectionofmembrane-boundtumornecrosisFactor(TNF):ananalysisofTNF-specificreagents.MicroscResTech2000,50:243 3.-Demjen,Detal;NeutralizationofCD95ligandpromotesregenerationandfunctionalrecoveryafterspinalcordinjury.NatMed2004,10:389 4.-Rajashekhar,Getal;Divergentandconvergenteffectsongeneexpressionandfunctioninacuteversuschronicendothelialactivation.PhysiolGenomics2007,31:104 5.-Sangaletti,Setal;Oncogene-drivenintrinsicinflammationinducesleukocyteproductionoftumornecrosisfactorthatcriticallycontributestomammarycarcinogenesis.CancerRes2010,70:7764 |
| StorageandstABIlity | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforatleastoneyear.Theexactexpirydateisindicatedonthelabel. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
| Disease | Autoimmunity,Gastroenterology |
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