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| AlternativeName: | CD4,GHS-R |
| Host: | Rabbit |
| Immunogen: | SyntheticpeptidecorrespondingtothesequenceneartheN-terminusofratGhrelinReceptor(GHS-R). |
| UniProtID: | O08725 |
| Speciesreactivity: | Rat |
| Crossreactivity: | Predictedspeciesreactivitybasedonepitopesequencehomology:human,mouse,marmoset,rhesusmacaque,platypus,horse. |
| Applications: | ELISA,IHC,WB |
| ApplicationNotes: | Suitableformembrane-basedELISA. PredictedMWof~41kDa.HigherMWspecies(~75kDa)andintermediatesalsoobservedbyWesternblotwhichreflectpost-translationalmodificationandheterodimerizationofthereceptor. |
| PurityDetail: | Peptideaffinitypurified. |
| Formulation: | Liquid.InPBScontaining50%glyceroland0.01%sodiumazide. |
| Handling: | Keeponiceatalltimes. |
| Shipping: | ShippedonBlueIce |
| LongTermStorage: | -20°C |
| ScientificBackground: | Ghrelinisamultifunctionalpeptidehormonesecretedfromtheanteriorpituitaryknowntoaffectmanyphysiologicalprocessesincludingthereleaseofgrowthhormone,appetiteregulation,gastricacidsecretion,gastrointestinalmotility,andbloodpressureregulation.The28aminoacidghrelinpeptideismodifiedatSer3byadditionofafattyacidgroup(i.e.,n-octanoyl)thatisessentialforitsbiologicalactivity.Theghrelinreceptor(GHS-R)isaseventransmembrane(7-TM)GPCRthatisexpressedinthehippocampus,pituitary,andhypothalamus,aswellasinperipheraltissuesincludingtheheart,lung,liver,kidney,pancreas,intestines,andimmunecells.GhrelinsignalsareprimarilyrelayedviacouplingoftheGHS-RtoGq/11alphasubunits,promotingstimulationofphospholipaseC,andanincreaseinintracellularcalcium.StimulationofadenylylcyclasesandincreasedcAMParealsoinducedbyGHS-Ractivation.Inhumansandswine,twosplicevariantsoftheghrelinreceptorareexpressedfromthesamegene.TheGHS-R1(a)receptoristhe366aminoacid,7-TMform,whileGHS-R1(b)isa289aminoacid,5-TMformthatistruncatedattheC-terminusandispharmacologicallyinactive.Theepitoperecognizedbythisantibodyisunaffectedbyalternativesplicing. |
| Manufacturer: | ManufacturedbyProteimax. |