产品资料

Rockland/Adenosine Deaminase Antibody Biotin Conjugated/200-406-140S/25 µL

AdenosineDeaminaseAntibodyBiotinConjugatedProperties

Description

Anti-ADENOSINEDEAMINASE(CalfSpleen)(RABBIT)AntibodyBiotinConjugated-200-406-140S

Host

Rabbit

TargetSpecies

Bovine

KnownCrossReactivity

BovineCalfspleen

Clonality

Polyclonal

Application

ELISA:1:5,000-1:20,000
WesternBlot:1:500-1:5,000
ImmunoPrecipitation:1:100
OtherDilution:UserOptimized

PhysicalState

Liquid(sterilefiltered)

ShippingCondition

DryIce

Format

IgG

Label

Biotin

Concentration

1.0mg/mLbyUVabsorbanceat280nm

Buffer

0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2

Preservative

0.01%(w/v)SodiumAzide

StABIlizer

10mg/mLBovineSerumAlbumin(BSA)-ImmunoglobulinandProteasefree

GeneName

ADA

RelevantLinks

UniProtKB-P56658
NCBI-P56658.3
GeneID-280712

AdenosineDeaminaseAntibodyBiotinConjugatedDescription

Background

AdenosineDeaminasecatalyzesthedeaminationofadenosinetoinosine,thusdestabilizingdouble-strandedRNA.Anti-AdenosineDeaminaseisamemberoftheadenosineandAMPdeaminasesfamily.Thisantibodyisafactorinpurinemetabolismandadenosinehomeostasis,aswellaspositivelyregulatingT-cellcoactivationthroughthebindingofDPP4.TheantibodyinteractswithDPP4intheextracellulardomain,thusregulatinglymphocyte-epithelialcelladhesion.Itmoderatesextracellularadenosinesignaling.EnzymedeficiencyinAdenosineDeaminasehasbeenassociatedwithseverecombinedimmunodeficiencydisease,whereasanoverabundancemayresultincongenitalhemolyticanemia.Anti-AdenosineDeaminaseisidealforinvestigatorsinvolvedinEnzyme,DNAandRNAresearch.

Synonyms

rabbitanti-AdenosineDeaminaseAntibodybiotinConjugation,biotinconjugatedrabbitanti-AdenosineDeaminaseAntibody,Adenosinedeaminase,Adenosineaminohydrolase

Immunogen

AdenosineDeaminase[CalfSpleen]

ImmunogenType

NativeProtein

StorageCondition

Storevialat-20°Corbelowpriortoopening.Thisvialcontainsarelativelylowvolumeofreagent(25µL).Tominimizelossofvolumedilute1:10byadding225µLofthebufferstatedabovedirectlytothevial.Recap,mixthoroughlyandbrieflycentrifugetocollectthevolumeatthebottomofthevial.Usethisintermediatedilutionwhencalculatingfinaldilutionsasrecommendedbelow.Storethevialat-20°Corbelowafterdilution.Avoidcyclesoffreezingandthawing.

ApplicationNote

Thisproducthasbeenassayedagainst1.0ugofAdenosineDeaminaseinastandardcaptureELISAusingPeroxidaseConjugatedStreptavidin#S000-03andABTS(2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid])code#ABTS-100asasubstratefor30minutesatroomtemperature. Aworkingdilutionof1:15,000to1:64,000ofthereconstitutionconcentrationissuggestedforthisproduct.

Purity/Specifity

ThisproductisanIgGfractionantibodypurifiedfrommonospecificantiserumbyamulti-stepprocesswhichincludesdelipidation,saltfractionationandionexchangechromatographyfollowedbyextensivedialysisagainstthebufferstatedabove. Assaybyimmunoelectrophoresisresultedinasingleprecipitinarcagainstanti-Biotin,anti-RabbitSerumaswellaspurifiedandpartiallypurifiedAdenosineDeaminase[CalfSpleen]. CrossreactivityagainstAdenosineDeaminasefromothersourcesmayoccurbuthasnotbeenspecificallydetermined.

DisclaimerNote-General

Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.

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Rockland/Adenosine Deaminase Antibody Biotin Conjugated/200-406-140S/25 µL

AdenosineDeaminaseAntibodyBiotinConjugatedProperties

AdenosineDeaminaseAntibodyBiotinConjugatedDescription