产品资料

Rockland/AKT pS473 Antibody/600-401-268S/25 µL

AKTpS473AntibodyProperties

Description

Anti-AKTpS473(RABBIT)Antibody-600-401-268S

Host

Rabbit

TargetSpecies

Human

KnownCrossReactivity

Human,Mouse,Rat

Clonality

Polyclonal

Application

ELISA:1:15,000-1:60,000
FlowCytometry:UserOptimized
WesternBlot:1:200-1:1000
Immunohistochemistry:1:100-1:500
OtherDilution:UserOptimized

PhysicalState

Liquid(sterilefiltered)

ShippingCondition

DryIce

Format

IgG

Label

Unconjugated

Concentration

1.0mg/mLbyUVabsorbanceat280nm

Buffer

0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2

Preservative

0.01%(w/v)SodiumAzide

StABIlizer

None

GeneName

AKT1

Paired

CellViability

RelevantLinks

NCBI-62241011
UniProtKB-P31749
GeneID-207

AKTpS473AntibodyDescription

Background

AKTisacomponentofthePI-3kinasepathwayandisactivatedbyphosphorylationat Ser473andThr308. AKTisacytoplasmicproteinalsoknownasAKT1,ProteinKinaseB(PKB)andrac(relatedtoAandCkinases).AKTisakeyregulatorofmanysignaltransductionpathways. AKTExhibitstightcontrolovercellproliferationandcellviability. OverexpressionorinappropriateactivationofAKTisnotedinmanytypesofcancer. AKTmediatesmanyofthedownstreameventsofPI3-kinase(alipidkinaseactivatedbygrowthfactors,cytokinesandinsulin).PI3-kinaserecruitsAKTtothemembrane,whereitisactivatedbyPDK1phosphorylation.Oncephos-phorylated,AKTdissociatesfromthemembraneandphos-phorylatestargetsinthecytoplasmandthecellnucleus. AKThastwomainroles:(i)inhibitionofapoptosis;(ii)promotionofproliferation.

Synonyms

rabbitanti-AKTpS473Antibody,AKT1phosphoS473,RAC-PK-alpha,ProteinkinaseB,PKB,C-AKT,RAC-alphaserine/threonine-proteinkinase,Proto-oncogenec-Akt,AKT1,AKT1,AKT-1

Immunogen

ThiswholerabbitserumwaspreparedbyrepeatedimmunizationswithasyntheticpeptidecorrespondingtoaC-terminusregionnearaa460-480ofhuman,mouse,ratandchickenAKTproteinsconjugatedtoKLH.

ImmunogenType

Peptide

PostTranslationalModification

Phosphorylation

StorageCondition

Storevialat-20°Corbelowpriortoopening.Thisvialcontainsarelativelylowvolumeofreagent(25µL).Tominimizelossofvolumedilute1:10byadding225µLofthebufferstatedabovedirectlytothevial.Recap,mixthoroughlyandbrieflycentrifugetocollectthevolumeatthebottomofthevial.Usethisintermediatedilutionwhencalculatingfinaldilutionsasrecommendedbelow.Storethevialat-20°Corbelowafterdilution.Avoidcyclesoffreezingandthawing.

ApplicationNote

ThisantibodyisphosphospecificforpS473andissuitableforwesternblotting,immunohistochemistry(formalin-fixedparaffin-embeddedsections),immunofluorescenceandELISA.Byimmunoblotasinglebandoftheexpectedapparentmolecularweightisobserved. Forimmunohistochemistrynopre-treatmentofsampleisrequired.

Purity/Specifity

Thisproductwaspreparedfrommonospecificantiserumbyimmunoaffinitychromatographyusingphosphopeptidecoupledtoagarosebeadsfollowedbysolidphaseadsorption(s)againstnon-phosphopeptideandnon-specificpeptidetoremoveanyunwantedreactivities.Assaybyimmunoelectrophoresisresultedinasingleprecipitinarcagainstanti-RabbitSerum. ThisantibodyisspecificforphosphorylatedhumanAKTpS473. Minimalreactivityoccursagainstnon-phosphorylatedAKT. ReactivityagainstAKTfromotherspeciesmayoccurbuthasnotyetbeentested.

DisclaimerNote-General

Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.

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Rockland/AKT pS473 Antibody/600-401-268S/25 µL

AKTpS473AntibodyProperties

AKTpS473AntibodyDescription