产品资料

Rockland/AKT1 APC Antibody/200-326-I51/50 µg

AKT1APCAntibodyProperties

Description

Anti-AKT1(MOUSE)AllophycocyaninConjugatedMonoclonalAntibody-200-326-I51

Host

Mouse

TargetSpecies

Human

KnownCrossReactivity

Human,Mouse,Rat

Clonality

Monoclonal14E5.A2.B2.H9IgG2akappa

Application

ELISA:UserOptimized
IFMicroscopy:Useroptimized
FlowCytometry:1.0µg/mL
WesternBlot:UserOptimized
Immunohistochemistry:Useroptimized

PhysicalState

Lyophilized

ShippingCondition

WetIce

Format

IgG2a

Label

Allophycocyanine

FPValue

1-2molesAllophycocyaninepermoleofIgG2a

Concentration

1.0mg/mLbyUVabsorbanceat280nm

Buffer

0.02MPotassiumPhosphate,0.5MSodiumChloride,pH7.2

ReconstitutionVolume

50µL

ReconstitutionBuffer

Restorewithdeionizedwater(orequivalent)

Preservative

None

StABIlizer

10mg/mLBovineSerumAlbumin(BSA)-ImmunoglobulinandProteasefree

GeneName

AKT1

RelevantLinks

UniProtKB-P31749
NCBI-NP_001014431.1
GeneID-207

AKT1APCAntibodyDescription

Background

AKTisacomponentofthePI-3kinasepathwayandisactivatedbyphosphorylationatSer473andThr308.AKTisacytoplasmicproteinalsoknownasAKT1,ProteinKinaseB(PKB)andrac(relatedtoAandCkinases).AKTisakeyregulatorofmanysignaltransductionpathways.AKTExhibitstightcontrolovercellproliferationandcellviability.OverexpressionorinappropriateactivationofAKTisnotedinmanytypesofcancer.AKTmediatesmanyofthedownstreameventsofPI3-kinase(alipidkinaseactivatedbygrowthfactors,cytokinesandinsulin).PI3-kinaserecruitsAKTtothemembrane,whereitisactivatedbyPDK1phosphorylation.Oncephosphorylated,AKTdissociatesfromthemembraneandphosphorylatestargetsinthecytoplasmandthecellnucleus.AKThastwomainroles:(i)inhibitionofapoptosis;(ii)promotionofproliferation.Anti-AKT1(MOUSE)APCconjugatedMonoclonalAntibodyisidealforinvestigatorsinvolvedinCellSignaling,Cancer,Neuroscience,SignalTransductionresearch.

Synonyms

mouseanti-AKT1antibodyAPCconjugation,allophycocyaninconjugatedmouseanti-AKT-1antibody,AKT-1,PKBantibody,PKBgammaantibody,PKBGAMMAantibody,PRKBGantibody,ProteinkinaseAkt1antibody,ProteinkinaseBgammaantibody,RAC-gammaserine/threonine-proteinkinase,RAC-PK-gamma

Immunogen

Anti-AKT1AntibodywasproducedinmicebyrepeatedimmunizationswithasyntheticpeptidecorrespondingtointernalresiduesofhumanAKT1proteinfollowedbymonoclonaldevelopment.

ImmunogenType

Peptide

StorageCondition

Storevialat4°Cpriortorestoration.Restorewithdeionizedwater(orequivalent).Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Forextendedstorage,aliquotcontentsandfreezeat-20°Corbelow.Avoidcyclesoffreezingandthawing.Storereagentinthedark.UsesuBDuedlightingduringhandlingandincubationofcellspriortoanalysis.

ApplicationNote

Anti-AKT1APCAntibodyissuitableforFlowCytometry,ELISA,immunohistochemistry,andwesternblotting.Expectabandapproximately56kDainsizecorrespondingtoAKT1proteinbywesternblottingintheappropriatecelllysateorextract.ThismonoclonalantibodyreactswithhumanAKT.Specificconditionsforreactivityshouldbeoptimizedbytheenduser.Forimmunohistochemistrywerecommendtheuseoffreshfrozentissues.Attemptsatstainingparaffin-embeddedformalinfixedtissueswerenegative.Nopre-treatmentofsampleisrequired.

Purity/Specifity

Anti-AKT1antibodyisdirectedagainsthumanAKT1.Theantibodydetectsbothunphosphorylatedandphosphorylatedformsoftheprotein.Anti-AKT1antibodywaspurifiedfromascitesbyProteinAchromatography.CrossreactivitywithAKT1fromotherspecieshasnotbeendetermined,however,thesequenceoftheimmunogenshows85%identitytomouseand92%identitywithrat,therefore,crossreactivityisexpected.

DisclaimerNote-General

Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.

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Rockland/AKT1 APC Antibody/200-326-I51/50 µg

AKT1APCAntibodyProperties

AKT1APCAntibodyDescription