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Sciencell/Recombinant Human B Cell Activating Factor /103-04/1 Ea

Thisproductisforresearchuseonly.Itisnotapprovedforuseinhumans,animals,orinvitrodiagnosticprocedures.
B-cellactivatingfactor(BAFF)isaTNFligandsuperfamilymemberandisproducedbymacrophages,dendriticcells,andTlymphocytes.BAFFpromotesthesurvivalofBcellsandisessentialforBcellmaturation.BAFFbindstothreeTNFreceptorsuperfamilymembers:B-cellmaturationantigen(BCMA/TNFRSF17),transmembraneactivatorandcalcium-modulatorandcyclophilinligandinteractor(TACI/TNFRSF13B)andBAFFreceptor(BAFFR/BR3/TNFRSF13C).ThesereceptorsaretypeIIItransmembraneproteinsthatlackasignalpeptide.WhereasTACIandBCMAbindBAFFandanotherTNFsuperfamilyligand,APRIL(aproliferation-inducingligand),BAFFRselectivelybindsBAFF.TheBAFFRextracellulardomainlackstheTNFreceptorcanonicalcysteine-richdomain(CRD)andcontainsonlyapartialCRDwithfourαysteineresidues.HumanandmouseBAFFRshare56%identity.BAFFRishighlyexpressedinspleen,lymphnodeandrestingBcells.ItisalsoexpressedatlowerlevelsinactivatedBcell,inrestingCD4+Tcells,inthymusandperipheralbloodleukocytes.

Specifications:

CatalogNo.103-04
CountryofManufactureUnitedStates
ProductCoderhBAFF
Size/Quantity5μg
ProductUseThisproductisforresearchuseonly.Itisnotapprovedforuseinhumans,animals,orinvitrodiagnosticprocedures.
StorageStoreat-20°Cafterreceiving.UponReconstitution,storeat2-8°Cforuptooneweek.FormaximalstABIlity,aliquotandstoreat-20°C.Avoidrepeatedfreeze/thawcycles.
ShippingInfoDryice
SynonymsBLys,TNFSF13B,TALL-1,CD257,THANK,andzTNF4
AASequenceMAVQGPEETVTQDCLQLIADSETPTIQKGSYTFVPWLLSFKRGSALEEKENKILVKETGYFFIYGQVLYTDKTYAMGHLIQRKKVHVFGDELSLVTLFRCIQNMPETLPNNSCYSAGIAKLEEGDELQLAIPRENAQISLDGDVTFFGALKLL
SourceEscherichiacoli
MolecularWeightApproximately17.0kDa,asinglenon-glycosylatedpolypeptidechaincontaining153aminoacids.
Purity>95%bySDS-PAGEandHPLCanalyses.
BIOLOGicalActivityTheED50is0.5-2ng/mLinthepresenceof10?g/mLofgoatantimouseIgMαchain,asdeterminedbycellproliferationassayusinganti-IgMstimulatedmouseBcells.
PhysicalAppearanceWhitelyophilizedpowder.
FormulationLyophilizedfroma0.2μmfilteredconcentrated(1mg/ml)solutioninPBS,pH7.0.
Endotoxin
ReconstitutionReconstituteinsteriledistilledwatercontaining0.1%BSAtoaconcentrationof0.1-1.0mg/mL.
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