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Sciencell/Recombinant Human Ciliary Neurotrophic Factor /107-08/1 Ea

Thisproductisforresearchuseonly.Itisnotapprovedforuseinhumans,animals,orinvitrodiagnosticprocedures.
Ciliaryneurotrophicfactor(CNTF)isapolypeptideinitiallypurifiedfromchickembryooculartissueandidentifiedasatrophicfactorforembryonicchickciliaryparasympatheticneuronsinculture.SubsequentstudieshavedemonstratedthatCNTFisasurvivalfactorforadditionalneuronalcelltypesincluding:dorsalrootganglionsensoryneurons,sympatheticganglionneurons,embryonicmotorneurons,majorpelvicganglionneuronsandhippocampalneurons.CNTFhasalsobeenshowntopreventthedegenerationofmotoraxonsafteraxotomy.ThegeneforhumanCNTFhasbeenlocalizedtotheproximalregionofthelongarmofchromosome11.TheCDNAforhumanCNTFencodesa200aminoacidresiduepolypeptidethatlacksasignalsequence.CNTFishighlyconservedacrossspeciesandexhibitscross-speciesactivities.HumanandratCNTFshareapproximately83%homologyintheirproteinsequence.CNTFisstructurallyrelatedtoIL-6,IL-11,LIFandOSM.Allofthesefourαelixbundlecytokinessharegp130asasignal-transducingsubunitintheirreceptorcomplexes.

Specifications:

CatalogNo.107-08
CountryofManufacture
ProductCoderhCNTF
Size/Quantity5μg
ProductUseThisproductisforresearchuseonly.Itisnotapprovedforuseinhumans,animals,orinvitrodiagnosticprocedures.
StorageStoreat-20°Cafterreceiving.UponReconstitution,storeat2-8°Cforuptooneweek.FormaximalstABIlity,aliquotandstoreat-20°C.Avoidrepeatedfreeze/thawcycles.
ShippingInfoDryice
AASequenceMAFTEHSPLTPHRRDLCSRSIWLARKLRSDLTALTESYVKHQGLNKNINLDSADGMPVASTDQWSELTEAERLQENLQAYRTFHVLLARLLEDQQVHFTPTEGDFHQAIHTLLLQVAAFAYQIEELMILLEYKIPRNEADGMPINVGDGGLFEKKLWGLKVLQELSQWTVRSIHDLRFISSHQTGIPARGSHYIANNKKM
SourceEscherichiacoli
MolecularWeightApproximately22.8kDa,asinglenon-glycosylatedpolypeptidechaincontaining199aminoacids.
Purity>97%bySDS-PAGEandHPLCanalyses.
BIOLOGicalActivityFullybiologicallyactive.TheED50is5x10^5,basedonthestimulationofTF-1cells.
PhysicalAppearanceWhitelyophilizedpowder.
FormulationLyophilizedfroma0.2μmfilteredconcentrated(1mg/ml)solutioninPBS,pH7.4.
Endotoxin
ReconstitutionReconstituteinsteriledistilledwatercontaining0.1%BSAtoaconcentrationof0.1-1.0mg/mL.
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