CatalogNo. | 125-07 |
CountryofManufacture | UnitedStates |
ProductCode | rMuVEGF165 |
Size/Quantity | 2μg |
ProductUse | Thisproductisforresearchuseonly.Itisnotapprovedforuseinhumans,animals,orinvitrodiagnosticprocedures. |
Storage | Thislyophilizedpreparationisstableat2-8°C,butshouldbekeptat-20°Cforlongtermstorage,preferablydesiccated.Uponreconstitution,thepreparationisstableforuptooneweekat |
ShippingInfo | Dryice |
AASequence | MAPTTEGEQKSHEVIKFMDVYQRSYCRPIETLVDIFQEYPDEIEYIFKPSCVPLMRCAGCCNDEALECVPTSESNITMQIMRIKPHQSQHIGEMSFLQHSRCECRPKKDRTKPEKHCEPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR |
Source | Escherichiacoli. |
MolecularWeight | RecombinantmurineVEGF165isa39.0kDadisulfide-linkedhomodimericproteinconsistingoftwo165aminoacidpolypeptidechains. |
Purity | >95%bySDS-PAGEandHPLCanalyses. |
BIOLOGicalActivity | Measuredbyitsabilitytostimulate3H-thymidineincorporationinHUVEcells.TheED50forthiseffectistypically2-4ng/mL. |
PhysicalAppearance | SterileFilteredWhitelyophilized(freeze-dried)powder. |
Formulation | Lyophilizedfroma0.2?mfilteredsolutioninPBS,pH7.4. |
Endotoxin | Lessthan1EU/μgofrmVEGF165asdeterminedbyLALmethod. |
Reconstitution | Werecommendthatthisvialbebrieflycentrifugedpriortoopeningtobringthecontentstothebottom.Reconstituteinsteriledistilledwateroraqueousbuffercontaining0.1%BSAtoaconcentrat |
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