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Sciencell/Recombinant Murine VEGF165/125-07/1 Ea

ThismaterialisofferedbyShanghaiPrimeGeneBio-Techforresearch,laboratoryorfurtherevaluationpurposes.NOTFORHUMANUSE.
VEGFwasinitiallypurifiedfrommediaconditionedbynormalbovinepituitaryfolliculo-stellatecellsandbyavarietyoftransformedcelllinesasamitogenspecificforvascularendothelialcells.ItwassubsequentlyfoundtobeidenticaltoanindependentlydiscoveredvascularpermeABIlityfactor(VPF),whichwaspreviouslyidentifiedinmediaconditionedbytumorcelllinesbasedonitsabilitytoincreasethepermeabilityofcapillarybloodvessels.ThreemouseCDNAclones,whicharisethroughalternativesplicingandwhichencodematuremousemonomericVEGFhaving120,164,or188,aminoacids,respectively,havebeenidentified.Tworeceptortyrosinekinases(RTKs),Flt-1andFlk-1(themousehomologueofhumanKDR),bothmembersofthetypeIIIsubclassofRTKscontainingsevenimmunoglobulin-likerepeatsintheirextracellulardomains,havebeenshowntobindVEGFwithhighaffinity.TherolesofthehomodimersofKDR,Flt,andtheheterodimerofKDR/FltinVEGFsignaltransductionremaintobeelucidated.Invivo,VEGFhasbeenfoundtobeapotentangiogenesisinducer.

Specifications:

CatalogNo.125-07
CountryofManufactureUnitedStates
ProductCoderMuVEGF165
Size/Quantity2μg
ProductUseThisproductisforresearchuseonly.Itisnotapprovedforuseinhumans,animals,orinvitrodiagnosticprocedures.
StorageThislyophilizedpreparationisstableat2-8°C,butshouldbekeptat-20°Cforlongtermstorage,preferablydesiccated.Uponreconstitution,thepreparationisstableforuptooneweekat
ShippingInfoDryice
AASequenceMAPTTEGEQKSHEVIKFMDVYQRSYCRPIETLVDIFQEYPDEIEYIFKPSCVPLMRCAGCCNDEALECVPTSESNITMQIMRIKPHQSQHIGEMSFLQHSRCECRPKKDRTKPEKHCEPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR
SourceEscherichiacoli.
MolecularWeightRecombinantmurineVEGF165isa39.0kDadisulfide-linkedhomodimericproteinconsistingoftwo165aminoacidpolypeptidechains.
Purity>95%bySDS-PAGEandHPLCanalyses.
BIOLOGicalActivityMeasuredbyitsabilitytostimulate3H-thymidineincorporationinHUVEcells.TheED50forthiseffectistypically2-4ng/mL.
PhysicalAppearanceSterileFilteredWhitelyophilized(freeze-dried)powder.
FormulationLyophilizedfroma0.2?mfilteredsolutioninPBS,pH7.4.
EndotoxinLessthan1EU/μgofrmVEGF165asdeterminedbyLALmethod.
ReconstitutionWerecommendthatthisvialbebrieflycentrifugedpriortoopeningtobringthecontentstothebottom.Reconstituteinsteriledistilledwateroraqueousbuffercontaining0.1%BSAtoaconcentrat
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