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Sciencell/Recombinant Rat Tumor Necrosis Factor-alpha /143-01/1 Ea

Thisproductisforresearchuseonly.Itisnotapprovedforuseinhumans,animals,orinvitrodiagnosticprocedures.
Tumornecrosisfactoralpha(TNF-α)isproducedbyneutrophils,activatedlymphocytes,macrophages,NKcells,LAKcells,astrocytesendothelialcells,smoothmusclecellsandsometransformedcells.TNF-αoccursasasecreted,solubleformandasamembrane-anchoredform,bothofwhichareBIOLOGicallyactive.Thenaturally-occurringformofTNF-αisglycosylated,butnon-glycosylatedrecombinantTNF-αhascomparable.ThebiologicallyactivenativeformofTNF-alphaisreportedlyatrimer.TwotypesofreceptorsforTNF-alphahavebeendescribedandvirtuallyallcelltypesstudiedshowthepresenceofoneorbothofthesereceptortypes.

Specifications:

CatalogNo.143-01
CountryofManufactureUnitedStates
ProductCoderrTNF-α
Size/Quantity5μg
ProductUseThisproductisforresearchuseonly.Itisnotapprovedforuseinhumans,animals,orinvitrodiagnosticprocedures.
StorageStoreat-20°Cafterreceiving.UponReconstitution,storeat2-8°Cforuptooneweek.FormaximalstABIlity,aliquotandstoreat-20°C.Avoidrepeatedfreeze/thawcycles.
ShippingInfoDryice
SynonymsTNF-alpha,Tumornecrosisfactorligandsuperfamilymember2,TNFa,Cachectin,DIF,TNFA,TNFSF2
AASequenceMLRSSSQNSSDKPVVHVVANHQAEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLIYSQVLFKGQGCPDYVLLTHTVSRFATSYQEKVSLLSAIKSPCPKDTPEGAELKPWYEPMYLGGVSQLEKGDLLSAEVNLPKYLDITESGQVYFGVIAL
SourceEscherichiacoli
MolecularWeightApproximately17.3kDa.asingle,non-glycosylatedpolypeptidechaincontaining157aminoacids.
Purity>95%bySDS-PAGEandHPLCanalyses.
BiologicalActivityFullybiologicallyactive.Specificactivity?5
PhysicalAppearanceWhitelyophilizedpowder.
FormulationLyophilizedfroma0.2μmfilteredconcentratedsolutionin20mMPB,pH7.2,150mMNaCl.
Endotoxin
ReconstitutionReconstituteinsteriledistilledwatercontaining0.1%BSAtoaconcentrationof0.1-1.0mg/mL.
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