Invitrogen™ C34777/Cholera Toxin Subunit B现货低价

Invitrogen™C34777/CholeraToxinSubunitB现货低价

100µg

Molecularprobes®choleratoxinconjugatesaremadefromarecombinantversionoftheBsubunitonly.Thisallowsustoprovideaveryhigh-purityproductthatiscompletelyfreeofthetoxicAsubunit.CholeratoxinBsubunit(CT-B)attachestocellsbybindingtogangliosideG_M1,makingitapowerfultoolforretrogradelabelingofneurons.Thistracerhasbeenusedinavarietyofapplications,includingtracingofratforebrainafferents,projectionsoftheparabrachialregion,andneuronsoftheurinarybladderwall.Whenusedinneuronaltracingapplications,CT-Bistypicallyintroducedbypressureinjectionorbyiontophoreticinjectionintoneuraltissue.

CholeraToxinSubunitBSpecifications:
•Label(Ex/Em):AlexaFluor®594(590/617nm)
•AtneutralpH,the11.4kDaBsubunitexistsasa57kDapentamer
•Lyophilizedproductcanbedissolvedinbuffer(e.g.,PBS)foruse

CholeraToxinSubunitBforStudyingLipidRafts
Morerecently,researchershavefoundthatCT-BcanbeusedasaMarkerforlipidrafts,whicharemembranemicrodomainsenrichedincholesterolandsphingolipidsthoughttobeimportantincellsignaling.Forlipidraftstaining,cellsarefirstincubatedwithfluorescentCT-B.Then,ananti–CT-BantibodyisaddedtocrosslinktheCT-Binthelipidraftsintodistinctpatchesontheplasmamembrane.Thesepatchesareeasilyvisualizedbyfluorescencemicroscopy.InadditiontoindividualfluorescentCT-Bconjugates,wealsoofferVybrant®LipidRaftLabelingKitsthatcontaintheAlexaFluor®488,AlexaFluor®555,orAlexaFluor®594dyeconjugatesofCT-B,ananti–CT-Bantibody,andadetailedprotocolforlabelingandpreparingcellsforfluorescencemicroscopy.

FindMoreCT-BConjugates
WeoffervariousCT-Bconjugates.ReviewProteinConjugates—Section14.7intheMolecularProbes®Handbookformore 

informationonthesetracers.

ForResearchUseOnly.Notforhumanoranimaltherapeuticordiagnosticuse.

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