AlphaSmooth Muscle Actin Polyclonal Antibody | Invitrogen PA519465 product information

homeInvitrogenAlpha-SmoothMuscleActinPolyclonalAntibody
otherbrands:NeoMarkers,LabVision,Endogen,Pierce,BioSourceInternational,ZymedLaboratories,Caltag,Molecularprobes,ResearchGenetics,LifeTechnologies,AppliedBiosystems,GibcoBRL,ABgene,Dynal,AffinityBioReagents,Nunc,Invitrogen,NatuTec,Oxoid,Richard-AllanScientific,Arcturus,PerseptiveBiosystems,Proxeon,eBioscienceproducttype:antibodyproductname:Alpha-SmoothMuscleActinPolyclonalAntibodycatalog:PA5-19465quantity:100µgprice:US390clonality:polyclonalhost:rabbitconjugate:nonconjugatedreactivity:human,mouse,rat,zebrafish,equineapplication:westernblot,immunohistochemistry,immunocytochemistry,immunohistochemistry-paraffinsection,immunohistochemistry-frozensectionmoreinfoororder:Invitrogenproductwebpage
PublishedApplication/Species/Sample/DilutionReferenceimmunohistochemistry-paraffinsection;mouse;1:100;loADIng...;fig4cimmunohistochemistry-paraffinsection;human;1:100;loading...;fig1ABIasinV,WygreckaM,MarshL,BeckerPaulyC,BrcicL,GhanimB,etal.Meprinβcontributestocollagendepositioninlungfibrosis.SciRep.2017;7:39969pubmedpublisher
immunocytochemistry;human;fig7HiguchiA,KaoS,LingQ,ChenY,LiH,AlarfajA,etal.Long-termxeno-freecultureofhumanpluripotentstemcellsonhydrogelswithoptimalelasticity.SciRep.2015;5:18136pubmedpublisher
westernblot;human;1:1000CaiT,ZhuW,ChenX,ZhouS,JiaL,SunY.Fibroblastgrowthfactor2inducesmesenchymalstemcellstodifferentiateintotenocytesthroughtheMAPKpathway.MolMedRep.2013;8:1323-8pubmedpublisher
immunohistochemistry;mouseFujimotoM,TsuneyamaK,FujimotoT,SelmiC,GershwinM,ShimadaY.Spirulinaimprovesnon-alcoholicsteatohepatitis,visceralfatmacrophageaggregation,andserumleptininamousemodelofmetabolicsyndrome.DigLiverDis.2012;44:767-74pubmedpublisher
PattanaikS,ArbraC,BainbridgeH,DennisS,FannS,YostM.VascularTissueEngineeringUsingScaffold-FreePrevascularEndothelial-FibroblastConstructs.BioresOpenAccess.2019;8:1-15pubmedpublisher
XieX,PercipalleP.Elevatedtransforminggrowthfactor?signalingactivationin?-actin-knockoutmouseembryonicfibroblastsenhancesmyofibroblastfeatures.JCellPhysiol.2018;:pubmedpublisher
ReebyeV,HuangK,LinV,JarvisS,CutilasP,DormanS,etal.GeneactivationofCEBPAusingsaRNA:preclinicalstudiesofthefirstinhumansaRNAdrugcandidateforlivercancer.Oncogene.2018;37:3216-3228pubmedpublisher
ToghillB,SaratzisA,FreemanP,SylviusN,BownM.SMYD2promoterDNAmethylationisassociatedwithaBDominalaorticaneurysm(AAA)andSMYD2expressioninvascularsmoothmusclecells.ClinEpigenetics.2018;10:29pubmedpublisher
NtokouA,SziborM,RodríguezCastilloJ,QuantiusJ,HeroldS,ElAghaE,etal.AnovelmouseCre-driverlinetargetingPerilipin2-expressingcellsintheneonatallung.Genesis.2017;55:pubmedpublisher
image1:WesternblotanalysisofMEF1WholeCellLysateusingPA5-19465,alphasmoothmuscleActinprimaryantibodyatadilutionof1ug/ml(lane1).PA5-19465stainingofRatBrainTissueLysateatadilutionof1ug/ml(lane2).BlottreatedwithasecondaryIRDye680-conjugatedGoatpolyclonalanti-Rabbitantibodywasusedatadilutionof1:10000.
image2:ImmunofluorescentstainingofHeLacellsusingPA5-19465,anti-AlphasmoothmuscleActinantibody.ThecellswerefixedwithPFA(4%)for10minutes,permabilisedwithBSA(1%),normalgoatserum(10%)andglycine(0.3M)in0.1%T-BSTfor20minutesandexposedtotheprimaryantibodyataconcentrationof10ug/mlfor1houratroomtemp.Thesecondaryantibodywasa448fluorescenceconjugatedGoatanti-rabbitIgG(green)atadilutionof1:1000.AWGA-594fluorescentconjugatedstainwasusedtolabelplasmamembranes(red)andthenucleistainwasDAPI(blue).
Applications:Immunohistochemistry(Frozen):1:1000,Immunohistochemistry(Paraffin):Assay-Dependent,Immunohistochemistry(PFAfixed):1:200,WesternBlot:1µg/mLSpecies:Equine,Human,Mouse,Rat,ZebrafishIsotype:Storage:-20°Cor-80°CifpreferredDescription:SmoothMuscleActinbelongstotheactinfamilyofproteins,whicharehighlyconservedproteinsthatplayaroleincellmotility,structureandintegrity.Alpha,betaandgammaactinisoformshavebeenidentified,withalphaactinbeingamajorconstituentofthecontractileapparatus,whilebetaandgammaactinsareinvolvedintheregulationofcellmotility.Inparticular,smoothmuscleactinisanalphaactinthatisfoundinskeletalmuscle.ActinexistsasaubiquitousproteininvolvedwithfilamentformationthatmakeuplargeportionsoftheCytoskeleton.Actinfilamentsinteractwithmyosintoassistinmusclecontractionaswellasaidingincellmotilityandcytokinesis.Smoothmuscleactinisfoundonsmoothmusclevesselwalls,gutwall,myometrium,myoepithelialcellsinbreastandsalivaryglands.Defectsinthesmoothmuscleactingenecauseaorticaneurysmfamilialthoracictype6.ActinisoformsdifferslightlyintheirN-terminusandthesequencesofeachareperfectlyconservedinhighervertebrates.Alpha-smoothmuscleactinisabundantinvascularandvisceralsmoothmusclecells.Inaddition,ithasalsobeenshownthatsmoothmuscleactinappearinstressfibersoffibroblasticcellsduringpathologicalsituationsinvolvingcontractilephenomenasuchaswoundhealingandfibrocontractivediseases.Multiplealternativelysplicedvariantsofsmoothmuscleactinhavebeenidentified.Immunogen:Syntheticpeptidederivedfromwithinresidues1-100ofHumanalphasmoothmuscleActin.Format:LiquidApplicationsw/Dilutions:Immunohistochemistry(Frozen):1:1000,Immunohistochemistry(Paraffin):Assay-Dependent,Immunohistochemistry(PFAfixed):1:200,WesternBlot:1µg/mLAliases:GIG46,AAT6,ACTSA,actin,aorticsmoothmuscle;alpha2actin;alpha-actin-2;alpha-cardiacactin;cellgrowth-inhibitinggene46protein;growth-inhibitinggene46;alpha-smamoreinfoororder:Invitrogenproductwebpage
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