IL2 Human ELISA Kit Invitrogen

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Pre-coated96wellplate
Standard
AssayDiluentconcentrate
BiotinylatedDetectionAntibody
SAV-HRP
WashBuffer
Chromogen
StopSolution
AdhesivePlateCovers

Aldesleukin,IL-2,T-cellgrowthfactor,TCGF,Interleukin-2,involvedinregulationofT-cellclonalexpansion,Tcellgrowthfactor

AboutThisKit

TheHumanInterleukin-2(HuIL-2)ELISAquantitatesHuIL-2inhumanserum,plasma,urine,orcellculturemedium.TheassaywillexclusivelyrecognizebothnaturalandrecombinantHuIL-2.

Principleofthemethod

TheHumanIL-2solid-phasesandwichELISA(enzyme-linkedimmunosorbentassay)isdesignedtomeasuretheamountofthetargetboundbetweenamatchedantibodypair.Atarget-specificantibodyhasbeenpre-coatedinthewellsofthesuppliedmicroplate.Samples,standards,orcontrolsarethenaddedintothesewellsandbindtotheimmobilized(capture)antibody.Thesandwichisformedbytheadditionofthesecond(detector)antibody,asubstratesolutionisaddedthatreactswiththeenzyme-antibody-targetcomplextoproducemeasurablesignal.Theintensityofthissignalisdirectlyproportionaltotheconcentrationoftargetpresentintheoriginalspecimen.

Rigorousvalidation

EachmanufacturedlotofthisELISAkitisqualitytestedforcriteriasuchassensitivity,specificity,precision,andlot-to-lotconsistency.Seemanualformoreinformationonvalidation.

Targetinformation

Interleukin2(IL-2)isanimmuno-modulatorycytokinethatisimportantfortheproliferationofactivatedTcells,differentiationofBcells,naturalkillercells,monocytesandmacrophages.IL-2signalsthroughtheIL-2receptor(IL-2R),aheterotrimericproteincomplexwhosegammachainisalsosharedbyinterleukin4(IL-4)andinterleukin7(IL-7).TheexpressionoftheIL-2geneinmaturethymocytesismonoallelic,whichrepresentsanunusualregulatorymodeforcontrollingthepreciseexpressionofasinglegene.ThetargeteddisruptionofagenesimilartoIL-2inmiceleadstoanulcerativecolitis-likediseasethatsuggestsanessentialroleofthisgeneintheimmuneresponsetoantigenicstimuli.

ForResearchUseOnly.Notforuseindiagnosticprocedures.Notforresalewithoutexpressauthorization.

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git-commit:fc3b4f69cd4415411ee445695f0cbc3e1ab21d31
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git-branch:origin/release/1.4.0-2020.05.19-1.0