IgA Mouse ELISA Kit Invitrogen

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Pre-coated96wellplate
Standard
AssayDiluentconcentrate
BiotinylatedDetectionAntibody
SAV-HRP
WashBuffer
Chromogen
StopSolution
AdhesivePlateCovers

AboutThisKit

TheMouseImmunoglobulinA(MsIgA)ELISAquantitatesMsIgAinmouseserum,plasma,orcellculturemedium.TheassaywillexclusivelyrecognizebothnaturalandrecombinantMsIgA.

Principleofthemethod

TheMouseIgAsolid-phasesandwichELISA(enzyme-linkedimmunosorbentassay)isdesignedtomeasuretheamountofthetargetboundbetweenamatchedantibodypair.Atarget-specificantibodyhasbeenpre-coatedinthewellsofthesuppliedmicroplate.Samples,standards,orcontrolsarethenaddedintothesewellsandbindtotheimmobilized(capture)antibody.Thesandwichisformedbytheadditionofthesecond(detector)antibody,asubstratesolutionisaddedthatreactswiththeenzyme-antibody-targetcomplextoproducemeasurablesignal.Theintensityofthissignalisdirectlyproportionaltotheconcentrationoftargetpresentintheoriginalspecimen.

Rigorousvalidation

EachmanufacturedlotofthisELISAkitisqualitytestedforcriteriasuchassensitivity,specificity,precision,andlot-to-lotconsistency.Seemanualformoreinformationonvalidation.

Targetinformation

Theisotypeofaprimaryantibodyandtheapplicationitisbeingusedincanresultinbackgroundstaining.PrimaryantibodybackgroundnoisecanbecausedbybindingtoFcreceptorsontargetcells;bynon-specificinteractionswithcellularproteins,carbohydrates,andlipids;orbycellautofluorescence.Isotypecontrolantibodiescanactasnegativecontrolstohelpdifferentiatenon-specificbackgroundsignalfromspecificantibodysignalbecausetheyhavenorelevantspecificitytoatargetantigen.Whileisotypecontrolsaremostcommonlyusedinflowcytometry,theyareusefulinotherapplicationssuchaschromatinimmunoprecipitation(ChIP),immunohistochemistry,andgelshifts.Isotypecontrolsshouldmatchwiththeprimaryantibodyspeciesandisotypesothatthelevelofspecificstainingbytheprimaryantibodymaybeaccuratelydetermined.Ifusingdirectlylabeledprimaryantibodies,theisotypecontrolworksbestifconjugatedwiththesamelabelasthetestantibody.

ForResearchUseOnly.Notforuseindiagnosticprocedures.Notforresalewithoutexpressauthorization.

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