invitrogen公司A11032抗体现货

订货请咨询苏州蚂蚁淘生物科技有限公司

手机：18915418616

QQ：3507623898、3464842061

Goatanti-MouseIgG(H+L)HighlyCross-AdsorbedSecondaryAntibody,AlexaFluor594

ProductDetails

TestedApplications

Dilution

FlowCytometry(Flow)

1-10µg/mL

Immunocytochemistry(ICC)

2µg/mL

Immunofluorescence(IF)

2µg/mL

PublishedApplications

Immunohistochemistry(Frozen)(IHC(F))

See5publicationsbelow

Immunohistochemistry(IHC)

See6publicationsbelow

Immunocytochemistry(ICC)

See7publicationsbelow

Immunohistochemistry(Paraffin)(IHC(P))

See1publicationbelow

MiscellaneousPubMed(MISC)

See191publicationsbelow

ProductSpecifications

SpeciesReactivity

Mouse

Host/Isotype

Goat/IgG

Class

Polyclonal

Type

SecondaryAntibody

Immunogen

GammaImmunoglobinsHeavyandLightchains

Conjugate

AlexaFluor®594

Excitation/EmissionProfile

Viewspectra

Form

Liquid

Concentration

2mg/mL

Purification

purified

Storagebuffer

PBS,pH7.5

Contains

5mMsodiumazide

Storageconditions

4°C,storeindark

RRID

AB_2534091

Target

IgG

CrossAdsorption

AgainstbovineIgG,goatIgG,rabbitIgG,ratIgG,humanIgGandhumanserum

AntibodyForm

WholeAntibody

ProductSpecificInformation

Tominimizecross-reactivity,thesegoatanti-mouseIgG(H+L)wholesecondaryantibodieshavebeenaffinitypurifiedandcross-adsorbedagainstbovineIgG,goatIgG,rabbitIgG,ratIgG,humanIgG,andhumanserum.Cross-adsorptionorpre-adsorptionisapurificationsteptoincreasespecificityoftheantibodyresultinginhighersensitivityandlessbackgroundstaining.Thesecondaryantibodysolutionispassedthroughacolumnmatrixcontainingimmobilizedserumproteinsfrompotentiallycross-reactivespecies.Onlythenonspecific-bindingsecondaryantibodiesarecapturedinthecolumn,andthehighlyspecificsecondariesflowthrough.Thebenefitsofthisextrastepareapparentinmultiplexing/multicolor-stainingexperiments(e.g.,flowcytometry)wherethereispotentialcross-reactivitywithotherprimaryantibodiesorintissue/cellfluorescentstainingexperimentswheretherearemaybethepresenceofendogenousimmunoglobulins.

AlexaFluordyesareamongthemosttrustedfluorescentdyesavailabletoday.Invitrogen™AlexaFluor594dyeisabright,red-fluorescentdyewithexcitationideallysuitedtothe594nmlaserline.Forstablesignalgenerationinimagingandflowcytometry,AlexaFluor594dyeispH-insensitiveoverawidemolarrange.Probeswithhighfluorescencequantumyieldandhighphotostabilityallowdetectionoflow-abundancebiologicalstructureswithgreatsensitivity.AlexaFluor594dyemoleculescanbeattachedtoproteinsathighmolarratioswithoutsignificantself-quenching,enablingbrighterconjugatesandmoresensitivedetection.Thedegreeoflabelingforeachconjugateistypically2-8fluorophoremoleculesperIgGmolecule;theexactdegreeoflabelingisindicatedonthecertificateofanalysisforeachproductlot.

Usingconjugatesolutions:Centrifugetheproteinconjugatesolutionbrieflyinamicrocentrifugebeforeuse;addonlythesupernatanttotheexperiment.Thisstepwillhelpeliminateanyproteinaggregatesthatmayhaveformedduringstorage,therebyreducingnonspecificbackgroundstaining.Becausestainingprotocolsvarywithapplication,theappropriatedilutionofantibodyshouldbedeterminedempirically.Forthefluorophore-labeledantibodiesafinalconcentrationof1-10µg/mLshouldbesatisfactoryformostimmunohistochemistryandflowcytometryapplications.

Background/TargetInformation

WeofferanextensivelineofInvitrogen™secondaryantibodyconjugateswithwell-characterizedspecificityandlabeledwithawideselectionofpremiumfluorescentdyes,includingInvitrogen™AlexaFluor™fluorescentdyes.Fluorescentsecondaryantibodyconjugatesareusefulinthedetection,sorting,orpurificationofitsspecifiedtargetandidealforfluorescencemicroscopyandconfocallaserscanningmicroscopy,flowcytometry,andfluorescentwesterndetection.ThebreadthoffluorescentMarkersweofferallowsourreagentstobetailoredtoalmostanyfluorescentdetectionsystem.

Secondaryantibodiesmaybeprovidedinthreeformats:wholeIgG,divalentF(ab')2fragments,andmonovalentFabfragments.Becauseofthehighdegreeofconservationinthestructureofmanyimmunoglobulindomains,mostclass-specificsecondaryantibodiesmustbeaffinity-purifiedandcross-adsorbedtoachieveminimalcross-reactionwithotherimmunoglobulins.

Oursecondaryantibodyconjugatesaremostcommonlypreparedbyimmunizingthehostanimalwithapooledpopulationofimmunoglobulinsfromthetargetspeciesandcanbefurtherpurifiedandmodified(e.g.,immunoaffinitychromatography,antibodyfragmentation,labelconjugation,etc.)togeneratehighlyspecificreagents.Inthefirstroundofpurification,wholeimmunoglobulinsbindingtotheimmunizingantibodyarerecoveredandmainlyconsistofthe~150-kDaIgGclass.Furtherpurification,forexample,withProteinAorG,removesallunwantedimmunoglobulinclassesexcepttheaffinity-purifiedantibodiesthatreactwiththetarget-specificimmunoglobulinheavyand/orlightchains.

ForResearchUseOnly.Notforuseindiagnosticprocedures.Notforresalewithoutexpressauthorization.