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Revmab/Anti-Acetyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody, Clone RM130; H3K14ac, Histone H3 (K14 acetylation)/31-1032188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Revmab/Anti-Acetyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody, Clone RM130; H3K14ac, Histone H3 (K14 acetylation)/31-1032

ProductDetails
ProductNameAnti-Acetyl-HistoneH3(Lys14)RabbitMonoclonalAntibody[RM130]
ProductDescriptionRabbitmonoclonaltoAcetylatedHistoneH3Lysine14(K14ac),H3K14ac,HistoneH3(K14acetylation)
CatalogNo.31-1032-00
CloneNameRM130
ProductDataSheetAnti-Acetyl-HistoneH3Lys14RabbitMonoclonalAntibodyDataSheetRM130
SpecificityRM130reactstoHistoneH3acetylatedatLysine14(K14ac),andisnotaffectedbythemodificationofneighboringaminoacids.NocrossreactivitywithacetylatedLysine4(K4ac),Lysine9(K9ac),Lysine18(K18ac),Lysine23(K23ac),Lysine27(K27ac),Lysine36(K36ac),orlysine79(K79ac)inhistoneH3.
ImmunogenAnacetyl-peptidecorrespondingtoAcetyl-HistoneH3(Lys14)
ApplicationWesternBlot(WB)
Immunocytochemistry(ICC)
ChromatinIP(ChIP)
ELISA
Multiplex
SpeciesReactivityHuman
Vertebrates
ConjugateNone
IntendedUseResearchUseOnly
.
Properties
FormLiquid
StorageInstructionsStoreat-20.0°C
StABIlityStablefor1Yearat-20.0°Cfromdateofreceipt
StorageBuffer50%Glycerol/PBSwith1%BSAand0.09%sodiumazide
Volume/Size100ug
Concentration1.0mg/mL
PurityProteinAaffinitypurifiedfromananimalorigin–freeculturesupernatant
CloneTypeMonoclonal
IsotypeRabbitIgG
UsageWesternBlot:0.5ug/mL-2ug/mL;
ICC:0.5ug/mL-2ug/mL;
ChIP:2ug/mL-10ug/mL;
ELISA:0.2ug/mL-1ug/mL;
Multiplex:0.1ug/mL–0.5ug/mL.
Keywords
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ProductImages
RM130

RM130specificallyreactstoHistoneH3acetylated
at Lysine 14 (K14ac). No cross reactivity with
acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine
18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac),
Lysine 36 (K36ac), or lysine 79 (K79ac) in histone H3.

The

ThebindingspecificityofRM130toHistoneH3
acetylated at Lysine 14 (K14ac) is not affected
by the modification of neighboring amino acids.

Western

WesternBlotofacidextractsfromHeLacells
untreated (-) or treated with sodium butyrate (+)
or, using RM130 at 0.5 ug/mL.

ChIP

ChIPperformedonHeLacellsusingH3K14ac
antibody (RM130, 5ug). Real-time PCR was
performed using primers specific to the gene
indicated.

Immunocytochemistry

ImmunocytochemistryofHeLacellstreatedwith
sodium butyrate, using Acetyl-Histone H3 (Lys14)
Rabbit mAb RM130 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).