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Revmab/Anti-Acetyl-Histone H3 (Lys18) Rabbit Monoclonal Antibody, Clone RM166; H3K18ac, Histone H3 (K18 acetylation)/31-1055-188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Revmab/Anti-Acetyl-Histone H3 (Lys18) Rabbit Monoclonal Antibody, Clone RM166; H3K18ac, Histone H3 (K18 acetylation)/31-1055-

ProductDetails
ProductNameAnti-Acetyl-HistoneH3(Lys18)RabbitMonoclonalAntibody[RM166]
ProductDescriptionRabbitmonoclonaltoAcetylatedHistoneH3Lysine18(K18ac),H3K18ac,HistoneH3(K18acetylation)
CatalogNo.31-1055-00
CloneNameRM166
ProductDataSheetAnti-Acetyl-HistoneH3Lys18RabbitMonoclonalAntibodyDataSheetRM166
SpecificityRM166reactstoHistoneH3acetylatedatLysine18(K18ac).NocrossreactivitywithotheracetylatedLysinesinhistoneH3.
ImmunogenAnacetyl-peptidecorrespondingtoAcetyl-HistoneH3(Lys18).
ApplicationWesternBlot(WB)
ChromatinIP(ChIP)
Immunocytochemistry(ICC)
Immunohistochemistry(IHC)
ELISA
Multiplex
SpeciesReactivityHuman
Vertebrates
ConjugateNone
IntendedUseResearchUseOnly
.
Properties
FormLiquid
StorageInstructionsStoreat-20.0°C
StABIlityStablefor1Yearat-20.0°Cfromdateofreceipt
StorageBuffer50%Glycerol/PBSwith1%BSAand0.09%sodiumazide
Volume/Size100ug
Concentration1.0mg/mL
PurityProteinAaffinitypurifiedfromananimalorigin–freeculturesupernatant
CloneTypeMonoclonal
IsotypeRabbitIgG
UsageWesternBlot:0.5ug/mL-2ug/mL;
ICC:0.5ug/mL-2ug/mL;
ChIP:2ug/mL-10ug/mL;
IHC:1ug/mL-10ug/mL;
ELISA:0.2ug/mL-1ug/mL;
Multiplex:0.1ug/mL–0.5ug/mL.

Keywords
ReferencesforAcetyl-HistoneH3(Lys18)antibody[RM166]LiY.etal.ActivationofmutantTERTpromoterbyRAS-ERKsignalingisakeystepinmalignantprogressionofBRAF-mutanthumanmelanomas.ProcNatlAcadSciUSA.113(50):14402-14407.ChIP;Human.Readmore(PubMed:27911794)
ProductImages
RM166

RM166specificallyreactstoHistoneH3
acetylated at Lysine 18 (K18ac). No cross reactivity
with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac),
Lysine 14 (K14ac), Lysine 23 (K23ac), Lysine 27
(K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac),
Lysine 79 (K79ac), or Lysine 122 (K122) in Histone H3.

Western

WesternBlotofacidextractsfromHeLacells
untreated (-) or treated with sodium butyrate (+),
using RM166 at 0.5 ug/mL, showed a band of
histone H3 acetylated at Lysine 18 in treated HeLa.

Sandwich

SandwichELISAagainstacetylatedhistoneH3
at Lys 18 using HeLa whole cell lysate, treated or
untreated with Sodium Butyrate. Using anti-H3CT
(RM188, 1 ug/mL) as the capture antibody and
biotinylated anti-H3K18C (RM166, 2 ug/mL) as the
detection antibody.

ChIP

ChIPperformedonHeLacellswithorwithout
Sodium Butyrate treatment, using H3K18Ac
antibody (RM166, 5ug). Real-time PCR was
performed using primers specific to the gene
indicated.

Immunocytochemistry

ImmunocytochemistryofHeLacellstreatedwith
sodium butyrate, using Acetyl-Histone H3 (Lys18)
Rabbit mAb RM166(red). Actin filaments have
been labeled with fluorescein phalloidin (green).

Immunohistochemistry

ImmunohistochemistrystainingofHepG2cellsusing
Anti-Acetyl-Histone H3 (Lys18) antibody, RM166.