ProductDetails | ||
ProductName | Anti-Acetyl-HistoneH3(Lys18)RabbitMonoclonalAntibody[RM166] | |
ProductDescription | RabbitmonoclonaltoAcetylatedHistoneH3Lysine18(K18ac),H3K18ac,HistoneH3(K18acetylation) | |
CatalogNo. | 31-1055-00 | |
CloneName | RM166 | |
ProductDataSheet | Anti-Acetyl-HistoneH3Lys18RabbitMonoclonalAntibodyDataSheetRM166 | |
Specificity | RM166reactstoHistoneH3acetylatedatLysine18(K18ac).NocrossreactivitywithotheracetylatedLysinesinhistoneH3. | |
Immunogen | Anacetyl-peptidecorrespondingtoAcetyl-HistoneH3(Lys18). | |
Application | WesternBlot(WB) ChromatinIP(ChIP) Immunocytochemistry(ICC) Immunohistochemistry(IHC) ELISA Multiplex | |
SpeciesReactivity | Human Vertebrates | |
Conjugate | None | |
IntendedUse | ResearchUseOnly | |
. | ||
Properties | ||
Form | Liquid | |
StorageInstructions | Storeat-20.0°C | |
StABIlity | Stablefor1Yearat-20.0°Cfromdateofreceipt | |
StorageBuffer | 50%Glycerol/PBSwith1%BSAand0.09%sodiumazide | |
Volume/Size | 100ug | |
Concentration | 1.0mg/mL | |
Purity | ProteinAaffinitypurifiedfromananimalorigin–freeculturesupernatant | |
CloneType | Monoclonal | |
Isotype | RabbitIgG | |
Usage | WesternBlot:0.5ug/mL-2ug/mL; ICC:0.5ug/mL-2ug/mL; ChIP:2ug/mL-10ug/mL; IHC:1ug/mL-10ug/mL; ELISA:0.2ug/mL-1ug/mL; Multiplex:0.1ug/mL–0.5ug/mL. | |
Keywords | ||
ReferencesforAcetyl-HistoneH3(Lys18)antibody[RM166] | LiY.etal.ActivationofmutantTERTpromoterbyRAS-ERKsignalingisakeystepinmalignantprogressionofBRAF-mutanthumanmelanomas.ProcNatlAcadSciUSA.113(50):14402-14407.ChIP;Human.Readmore(PubMed:27911794) | |
ProductImages | ||
RM166specificallyreactstoHistoneH3 | ||
WesternBlotofacidextractsfromHeLacells | ||
SandwichELISAagainstacetylatedhistoneH3 | ||
ChIPperformedonHeLacellswithorwithout | ||
ImmunocytochemistryofHeLacellstreatedwith | ||
ImmunohistochemistrystainingofHepG2cellsusing
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