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Revmab/Anti-Dimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody, Clone RM151; H3K9me2, Histone H3 (dimethyl K9)/31-1059-00-188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Revmab/Anti-Dimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody, Clone RM151; H3K9me2, Histone H3 (dimethyl K9)/31-1059-00-

ProductDetails
ProductNameAnti-Dimethyl-HistoneH3(Lys9)RabbitMonoclonalAntibody[RM151]
ProductDescriptionRabbitmonoclonaltoDimethylatedHistoneH3Lysine9,H3K9me2,HistoneH3(dimethylK9)
CatalogNo.31-1059-00
CloneNameRM151
ProductDataSheetAnti-Dimethyl-HistoneH3(Lys9)RabbitMonoclonalAntibodyDataSheetRM151
SpecificityRM151reactstoHistoneH3dimethylatedatLysine9(K9me2).NocrossreactivitywithmonomethylatedLysine9(K9me1),trimethylatedLysine9(K9me3),orothermethylationsinhistoneH3.
ImmunogenAdimethyl-peptidecorrespondingtoDimethyl-HistoneH3(Lys9).
ApplicationWesternBlot(WB)
ChromatinIP(ChIP)
Immunocytochemistry(ICC)
ELISA
Multiplex
SpeciesReactivityHuman
Vertebrates
ConjugateNone
IntendedUseResearchUseOnly
.
Properties
FormLiquid
StorageInstructionsStoreat-20.0°C
StABIlityStablefor1Yearat-20.0°Cfromdateofreceipt.
StorageBuffer50%Glycerol/PBSwith1%BSAand0.09%sodiumazide
Volume/Size100ug
Concentration1.0mg/mL
PurityProteinAaffinitypurifiedfromananimalorigin–freeculturesupernatant
CloneTypeMonoclonal
IsotypeRabbitIgG
UsageWesternBlot:0.25ug/mL–1ug/mL;
ChIP:2ug/mL-10ug/mL;
ICC:0.5ug/mL-2ug/mL;
ELISA:0.2ug/mL-1ug/mL;
Multiplex:0.1ug/mL–0.5ug/mL.

Keywords
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ProductImages
RM151

RM151specificallyreactstoHistoneH3dimethylated
at Lysine 9 (K9me2). No cross reactivity with non
-modified Lysine 9 (H3 1-19), monomethylated
Lysine 9 (K9me1), trimethylated Lysine 9
(K9me3), or other methylations in Histone H3.

Western

WesternBlotofrecombinanthistoneH3.3(1)
and acid extracts of HeLa cells (2), using RM151
at 0.5 ug/mL, showed a band of histone H3
dimethylated at Lysine 9 (K9me2) in HeLa cells.

ChIP

ChIPperformedonHeLacellsusingH3K9me2
antibody (RM151, 5ug). Real-time PCR was
performed using primers specific to the gene
indicated.

Immunocytochemistry

ImmunocytochemistryofHeLacellstreatedwith
sodium butyrate, using Dimethyl-Histone H3
(Lys9) Rabbit mAb RM151 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).