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Revmab/Anti-Histone H3 pan Rabbit Monoclonal Antibody, Clone RM188/31-1057-00/100ug

ProductDetails
ProductNameAnti-HistoneH3panRabbitMonoclonalAntibody[RM188]
ProductDescriptionRabbitmonoclonaltoHistoneH3pan
CatalogNo.31-1057-00
CloneNameRM188
ProductDataSheetAnti-HistoneH3panRabbitMonoclonalAntibodyDataSheetRM188
SpecificityRM188reactstoallHistoneH3proteins,independentofpost-translationalmodifications.Nocrossreactivitywithotherhistoneproteins
ImmunogenApeptidecorrespondingtotheC-terminusofhumanHistoneH3.
ApplicationWesternBlot(WB)
Immunocytochemistry(ICC)
ELISA
Multiplex
SpeciesReactivityHuman
Vertebrates
ConjugateNone
IntendedUseResearchUseOnly
.
Properties
FormLiquid
StorageInstructionsStoreat-20.0°C
StABIlityStablefor1Yearat-20.0°Cfromdateofreceipt
StorageBuffer50%Glycerol/PBSwith1%BSAand0.09%sodiumazide
Volume/Size100ug
Concentration1.0mg/mL
PurityProteinAaffinitypurifiedfromananimalorigin–freeculturesupernatant
CloneTypeMonoclonal
IsotypeRabbitIgG
UsageWesternBlot:0.01ug/mL–0.25ug/mL;
ICC:1ug/mL-5ug/mL;
ELISA:0.2ug/mL-1ug/mL;
Multiplex:0.1ug/mL–0.5ug/mL.


Keywords
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ProductImages
RM188

RM188reactstobothHistoneH3.1andHistoneH3.3.

Western

WesternBlotofrecombinantHistoneH3.3and
Histone H3.1 proteins, A375, HEK293, HeLa
and SK-MEL-2 whole cell lysates, using RM188
at 0.025 ug/mL, showed a band of recombinant
H3.1 and H3.3 proteins, and total histone
H3 in A375, HEK293, HeLa and SK-MEL-2 cells.

Immunocytochemistry

ImmunocytochemistryofHeLacells,usingHistone
H3 Rabbit mAb RM188 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).

Sandwich

SandwichELISAagainstacetylatedhistoneH3atLys9
using HeLa whole cell lysate, treated or untreated with
Sodium Butyrate. Using anti-H3CT (RM188, 1 ug/mL) as the
capture antibody and biotinylated anti-H3K9ac (RM161,
1 ug/mL) as the detection antibody.

Sandwich

SandwichELISAagainstacetylatedhistoneH3
at Lys 79 using HeLa whole cell lysate, treated or
untreated with Sodium Butyrate. Using anti-H3K79ac
(RM156, 5 ug/mL) as the capture antibody and
biotinylated anti-H3CT (RM188, 1 ug/mL) as the
detection antibody.