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Dog Heart mRNA (Poly A+ RNA) is purified from total RNA extracted from freshly harvested tissues using classical guanidine isothiocyanate–phenol:chloroform extraction method and treated with DNase to remove residual DNA contamination. Each poly A+ RNA sample is enriched for mRNA transcripts with two rounds of oligo(dT)-cellulose purification, precisely quantified, and stored at -80oC.
Quality Control: The integrity of each RNA sample is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by spectrophotometer (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR.
Applications: RNA is ideal for investigation of rare transcript using qPCR or Northern blot analysis, preparation of cDNA for RT-PCR/RACE analyses, and expression standard in microarray applications.
Packing/shipping: Total RNA sample is routinely provided in RNase-free water at a concentration of 0.25mg/ml or 0.5mg/ml and shipped on dry ice. Sample can also be provided in 1mM sodium citrate, 0.1 mM EDTA, or any other RNA storage buffer. Samples are shipped on dry ice.
MSDS and Certificate of Analysis in PDF files: Contact Zyagen Technical Support at zinfo@zyagen.com
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