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Zyagen/Monkey Brain mRNA, Cynomolgus/6848/1 Ea

MonkeyBrain,CynomolgusmRNA(PolyA+RNA)ispurifiedfromtotalRNAextractedfromfreshlyharvestedtissuesusingclassicalguanidineisothiocyanate–phenol:chloroformextractionmethodandtreatedwithDNasetoremoveresidualDNAcontamination.EachpolyA+RNAsampleisenrichedformRNAtranscriptswithtworoundsofoligo(dT)-cellulosepurification,preciselyquantified,andstoredat-80oC.

QualityControl: TheintegrityofeachRNAsampleisverifiedbydenaturedagarosegelelectrophoresis.ThepurityofRNAisassessedbyspectrophotometer(A260/A280:1.9-2.1). ResidualDNAcontaminationistestedbyPCR.

Applications: RNAisidealforinvestigationofraretranscriptusingqPCRorNorthernblotanalysis,preparation ofCDNAfor RT-PCR/RACEanalyses,andexpressionstandardinmicroarrayapplications.

Packing/shipping:TotalRNAsampleisroutinelyprovidedinRNase-freewaterataconcentrationof0.25mg/mlor0.5mg/mlandshippedondryice.Samplecanalsobeprovidedin1mMsodiumcitrate,0.1mMEDTA,oranyotherRNAstoragebuffer.Samplesareshippedondryice.

MSDSandCertificateofAnalysisinPDFfiles: ContactZyagenTechnicalSupportat zinfo@zyagen.com

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