BovineTestisfulllengthtissueCDNAismadeofRNAextractedfromfreshlyharvestedtissuesofsinglehealthynormaldonorusingclassicalguanidineisothiocyanate-phenol:chloroformextractionmethod. RNA istreatedwithRNase-freeDNase-1toremoveresidualDNA.ThecDNAisprimedwitholigodTprimer.
QualityControl:TheintegrityofeachRNAsampleasindicatedbyintactribosomalRNAisverifiedbydenaturedagarosegelelectrophoresis.ThepurityofRNAisassessedbyspectrophotometer(A260/A280:1.9-2.1).ResidualDNAcontaminationistestedbyPCR.
ThesynthesizedcDNAisalsotestedastemplateforPCRamplificationofß-actingene.PCRproductofß-actinwasvisualizedon1%agarosegel.
Applications:cDNAisidealforgeneexpressionanalysisbyPCR,characterizationofalternativesplicingofmRNA,verificationofgeneticmutation,genecloningandtargetsequencing.
Packing/shipping:eachcDNAsampleisroutinelyshippedondryicein1.5mlvialsandisenoughfor30reactions(30PCRamplifications).
MSDSandCertificateofAnalysisinPDFfiles:ContactZyagenTechnicalSupportatzinfo@zyagen.com
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn