ProductName | Alpha-Amylase,DFPTreated |
Source | PorcinePancreas |
CatalogueNumber | 122-18 |
Purity | Purified |
Form | Lyophilized |
ProductDetails | Alpha-Amylase(122-10)wastreatedwithDFP(diisopropylfluorophosphate)toinactivateserineproteases. |
Activity | >70U/mg(Dimension®ClinicalChemistrySystem) |
Protein | >0.4mgprotein/mg(Coomassie) |
SpecificActivity | >100U/mgprotein |
Contaminants | Lipase:5% Protease:1% Ammonia:0.1micromole/mg |
UnitDefinition | Oneunitwillcatalyzethehydrolysisofonemicromoleof2-chloro-4-nitrophenyl-a-D-maltotriosidetoyield2-chloro-4-nitrophenolperminuteat37°C. |
Formulation | Lyophilizedfrom20mMtris,150mMsodiumchloride,and3mMcalciumchloride,pH7.5 |
Appearance | Off-whitetowhitepowder |
Storage | -20°C |
Recertification | 3years |
Synonyms | 1,4-alpha-D-Glucan-glucanohydrolase,amy2,PPA,PA |
MolecularWeight | ~54,000 |
CASNumber | 9000-90-2 |
E.C.Number | 3.2.1.1 |
Gene | AMY2 |
GeneID | 397397 |
AccessionNo | P00690 |
LeeBiosolutionsproducesporcinepancreaticalpha-Amylaseforclinicalresearchanddiagnosticmanufacturing.
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UnitDefinition:Oneunitwillcatalyzethehydrolysisofonemicromoleofmaltopentaose,whichthroughcoupledreactionsresultsintheformationof5micromolesofglucose=6-phosphateperminuteat37C.Measuredat340nmasanequimolaramountofNADHproducedbyacoupledreaction.
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