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Lee Biosolutions/Alpha-Amylase - DFP Treated/122-18-5/5 kU

Description
ProductNameAlpha-Amylase,DFPTreated
SourcePorcinePancreas
CatalogueNumber122-18
PurityPurified
FormLyophilized
ProductDetailsAlpha-Amylase(122-10)wastreatedwithDFP(diisopropylfluorophosphate)toinactivateserineproteases.
Activity>70U/mg(Dimension®ClinicalChemistrySystem)
Protein>0.4mgprotein/mg(Coomassie)
SpecificActivity>100U/mgprotein
ContaminantsLipase:5%
Protease:1%
Ammonia:0.1micromole/mg
UnitDefinitionOneunitwillcatalyzethehydrolysisofonemicromoleof2-chloro-4-nitrophenyl-a-D-maltotriosidetoyield2-chloro-4-nitrophenolperminuteat37°C.
FormulationLyophilizedfrom20mMtris,150mMsodiumchloride,and3mMcalciumchloride,pH7.5
AppearanceOff-whitetowhitepowder
Storage-20°C
Recertification3years
Synonyms1,4-alpha-D-Glucan-glucanohydrolase,amy2,PPA,PA
MolecularWeight~54,000
CASNumber9000-90-2
E.C.Number3.2.1.1
GeneAMY2
GeneID397397
AccessionNoP00690
Applications
Controls/Calibrators/StandardsImmunoassaysClinicalChemistryAssayDevelopment/Validation
Overview

LeeBiosolutionsproducesporcinepancreaticalpha-Amylaseforclinicalresearchanddiagnosticmanufacturing.

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UnitDefinition:Oneunitwillcatalyzethehydrolysisofonemicromoleofmaltopentaose,whichthroughcoupledreactionsresultsintheformationof5micromolesofglucose=6-phosphateperminuteat37C.Measuredat340nmasanequimolaramountofNADHproducedbyacoupledreaction.

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