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Kapa biosystems/Library Preparation for Illumina/KK8710/24 adapters x 40 µl each

Description:

KAPASingle-IndexedAdapterKit,SetA+B(1.5µM)

LibraryPreparationforIllumina

It’scomplex,butwehaveitcovered.

KAPADNALibraryPreparationKitsforIllumina®sequencingplatformscontainevolvedandoptimallyformulatedenzymesthatenablethehighestoverallcoveragefromtheleastamountoftotalsequencing.

KitsareoptimizedtoachievesignificantlyhigherlibraryyieldsandcontainKAPAHiFiforhigh-fidelity,high-efficiencyandlow-biaslibraryamplification.Thisensuresthehighestlibraryandsequencedataquality,particularlyfromlow-inputanddifficultsamplessuchasFFPEandChIPDNA.

NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.

DownloadourKAPA AdapterandBeadCalculator

 

ForResearchUseOnly.Notforuseindiagnosticprocedures.

ProductHighlights

Achievegreatermolecularcomplexity

  • Higheryieldsofadapter-ligatedlibrarytranslatestohighermolecularcomplexity
  • Fewercyclesofamplificationareneeded,whichresultsinlowerPCRduplicationrates
  • PCR-freeworkflowspossIBLefrominputs≥100ng*

Reduceamplificationbiasandimprovecoverage

  • KAPAHiFireducesPCRbias*
  • ImprovescoverageuniformityofGC-andAT-richregions,promoters,lowcomplexityandotherchallengingregions*

Createhigh-qualitylibrariesfromchallengingsamples

  • High-qualitywholeexomesequencingfromlibrariespreparedusingaslittleas10nghumangenomicDNA*
  • Highsuccessrateswith250ngFFPEorless*
  • Routinelibraryconstructionfrom≥100pgChIPDNA*
KAPA Library Quantification methods (96- and 384-well) format are available for the Sciclone NGS and NGSx workstation (PerkinElmer) and other instruments used in high-throughput NGS sample preparation pipelines.

Automatewithpre-validatedscripts

 

*Dataonfile.

Applications:

Applications
  • WholeGenomeSequencing
  • ChIPSequencing
  • WholeExomeSequencing
  • AmpliconSequencing
  • TargetedSequencing(Capture)
  • MethylSequencing

KitSpecificationsandContents/Storage:

KitSpecificationsandContents/Storage

Kitscanbestoredforupto18months at-20˚C.

Kitsincludereagentsforendrepair,A-tailing,ligationandlibraryamplification.PCR-freeversions(withoutlibraryamplificationreagent)arealsoavailable.“With-bead”kitsincludePEG/NaClsolutionforSPRIbeadcleanups.
All96-librarykitsareautomationfriendly.

Components

Specifications

Spec
Description
CompatiblePlatform
IlluminaHiSeq,MiSeq,NextSeqandGAIIx
LibraryType
DNA
StartingMaterial
FragmentedDNAorPCRamplicons
InputAmount
100pg–5µg
SequencingApplications
WholeGenomeSequencingWholeExomeSequencingTargetedSequencing(custompanels)ChIP-SeqAmpliconSequencingMethyl-Seq
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