产品资料

Kapa biosystems/KAPA HyperPlus Kits/KK8721/25 mL

Description:

KAPAAdapterDilutionBuffer(25mL)

KAPAHyperPlusKits

NGSlibrarypreparation. Evolved.

TheKAPAHyperPluskitprovidesastreamlinedworkflowthatincludesfragmentationandlibrarypreparationinasingletube.Buildingonindustry-leADInglibraryconstructionefficiencies,thisintegratedsolutioncombinesenzymaticfragmentation,similarinqualitytomechanicalshearing,withthespeedandconvenienceoftagmentation-basedworkflows.Benefitsinclude:

DNAfragmentationandlibraryprepin2.5hours*
FlexIBLeDNAsampleinputfrom1ng–1µg*
Reducedbiasandmaximizesequencecoverage*
PCR-freeworkflows

NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.

Downloadour AdapterandBeadCalculator

^*Dataonfile.
ForResearchUseOnly.Notforuseindiagnosticprocedures.

KAPAHyperPlusWorkflow

ProductHighlights

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Tunableandreproduciblefragmentation

Adjustlibraryinsertsizesfrom150–800bpbyvaryingfragmentationtime
ReproducibleinsertsizesacrossarangeofGCcontentandDNAinputamounts

Industryleadinglibraryyields…

Routinelyachieveconversionrates>80%from≥100nginput*
SuperiorperformanceacrossarangeofDNAinputamounts*
HighlibraryyieldsenablePCR-freeworkflowsfromaslittleas50ngstartingmaterial*

Single nucleotide polymorphism (SNP) detection sensitivity indicates better sequencing quality. Libraries were prepared for capture using the Nimblegen SeqCap EZ HGSC VCRome panel, 50 ng input, standard capture protocol. SNPs were called using LoFreq with default settings and a minimum per-base depth of coverage cut-off of 10. Sensitivity (true positive rate) was calculated as the ratio of true positives divided by the sum of true positives and false negatives. Total SNPs analysed was greater than 25,000. Data on file.

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…Enablesuperiorsequencingresults

Higherconversionratesresultsinfeweramplificationcyclesandlowerduplicationrates
Detectlow-frequencymutationswithhighconfidenceduetogreaterlibrarydiversityandmoreuniformsequencecoverage

GC bias comparison. GC bias for C. difficile, E. coli, B. pertussis was assessed by calculating the GC content of the reference in 100 bp bins and plotting normalized coverage across these bins for Covaris and Hyper Prep, HyperPlus, NEBNext ® Ultra ™ and Nextera ™ workflows, using Picard CollectGCBiasMetrics. Libraries were prepared from 1 ng of input DNA. In the absence of sequencing bias, all bins would be equally represented, indicated by a horizontal distribution centered on a normalized coverage of 1.0. Distribution of GC content in the genome is indicated by the grey histograms. Data on file.

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Minimalsequencecoveragebias

Lowersequencebiaswhencomparedtotagmentationandotherenzymaticfragmentationmethods*
Equivalentperformancetomechanicalshearing*
Lessbiasleadstomoreuniformsequencingcoverageandreducedsequencingcosts*

_*Dataonfile.

RelatedProducts

Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:

KAPAhgDNAQuantificationandQCKit

KAPALibraryAmplificationKits

KAPALibraryQuantificationKits

KAPAStrandedmRNA-SeqKits

Applications:

Applications

WholeGenomeSequencing
WholeExomeSequencing
TargetedSequencing(custompanels)
FFPESampleSequencing

KitSpecificationsandContents/Storage:

KitSpecificationsandContents/Storage

Kitscanbestoredforupto6monthsat-20ºC.

KitscontainKAPAFragEnzyme,KAPAFragBuffer(10X),KAPAFragConditioningSolution,KAPAEndRepair&A-TailingBuffer, KAPAEndRepair&A-TailingEnzyme, KAPALigationBuffer, KAPADNALigase, KAPAHiFiHotStartReadyMixand KAPALibraryAmplificationPrimerMix.

Specifications

CompatiblePlatform

lluminaHiSeq®,MiSeq®,NextSeq®

LibraryType

DNA

StartingMaterial

genomicDNA,FFPEDNA,PCRamplicons

InputAmount

1ng–1µg

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Kapa biosystems/KAPA HyperPlus Kits/KK8721/25 mL

Description:

KAPAHyperPlusKits

NGSlibrarypreparation. Evolved.

ProductHighlights

Tunableandreproduciblefragmentation

Industryleadinglibraryyields…

…Enablesuperiorsequencingresults

Minimalsequencecoveragebias

RelatedProducts

Applications:

KitSpecificationsandContents/Storage:

Specifications