产品资料

Kapa biosystems/KAPA Stranded mRNA-Seq Kits/KK8711/12 adapters x 40 µl each

Description:

KAPASingle-IndexedAdapterKit,SetA(1.5µM)

KAPAStrandedmRNA-SeqKits

Evendifficultmessagesshouldbeunderstood.

KAPAStrandedmRNA-SeqKitsincludealltheenzymesandbuffersrequiredforCDNAlibrarypreparationforIlluminaNext-GenerationSequencing,utilizing100ng–4µgoftotalRNA.KAPAmRNACaptureBeadsareincludedforisolationofpoly(A)-tailedRNA.Kitsprovideprecisemeasurementofstrandorientation(>99%),uniformcoverage,andhigh-confidencemappingofalternatetranscripts,andareoptimizedfortheimprovedcoverageofGC-richandlow-abundancetranscripts.*KitscontainKAPAHiFiforhigh-efficiencyandlow-biaslibraryamplification,aswellasKAPAmRNACaptureBeadsandastreamlined,“with-bead”protocol.

KAPAStrandedRNA-SeqKitsincludealltheenzymesandbuffersrequiredforcDNAlibrarypreparationforIlluminaNext-GenerationSequencing,butdonotcontaintheKAPAmRNACaptureBeads.Kitscanbeusedtopreparelibrariesfrom10–400ngofeitherpoly(A)-selected,ribosomallydepleted,ortotalRNA.

NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.

Download AdapterandBeadCalculator

_*Dataonfile.ForResearchUseOnly.Notforuseindiagnosticprocedures.

ProductHighlights

Improved coverage of GC-rich transcripts. The 5

Uncoverchallengingtranscripts

ImprovedcoverageofGC-richtranscripts
Enhancedidentificationofexonicregions

Improved coverage of low-abundance transcripts. GLTPD1, a lesser-expressed transcript, is covered more comprehensively with the KAPA Stranded mRNA-Seq Kit (green) at 500 ng input total RNA (outlined in red). In comparison, Illumina TruSeq Stranded mRNA Sample Prep Kit (orange) shows coverage gaps, even with higher inputs (4 μg). Data on file.

Detectlow-abundancetranscripts

Enablesidentificationoftranscriptsmissedbycompetitorkits,evenwithhighinput*
Highuniformityacrossvaryingamountsofsampleinput*

30M),KAPAStrandedmRNA-SeqlibrariesgenerateagreaterpercentageofmappedreadsandlowerduplicationratesthananalogouslibrariespreparedwiththeIlluminaStrandedmRNASamplePrepKitwhilemaintaining99%strandspecificity.Dataonfile."> Highly mapped reads and strand specificity enable sensitive detection of expressed genes. With similar numbers of filter-passed reads (>30M),KAPAStrandedmRNA-SeqlibrariesgenerateagreaterpercentageofmappedreadsandlowerduplicationratesthananalogouslibrariespreparedwiththeIlluminaStrandedmRNASamplePrepKitwhilemaintaining99%strandspecificity.Dataonfile.

Highly mapped reads and strand specificity enable sensitive detection of expressed genes. With similar numbers of filter-passed reads (>30M),KAPAStrandedmRNA-SeqlibrariesgenerateagreaterpercentageofmappedreadsandlowerduplicationratesthananalogouslibrariespreparedwiththeIlluminaStrandedmRNASamplePrepKitwhilemaintaining99%strandspecificity.Dataonfile.

Identifymoregenes

HigherpercentageofuniquelymappedreadscomparedtoIllumina TruSeq™StrandedmRNASamplePrepKits*
Lowerduplicationratesyieldbettercoverage

Minimal positional bias. With intact, high-quality RNA input, 3

Maintainhighcoverageuniformity

Minimal5’–3′biasacrosstranscripts
Moreuniformdistributionofreadsovereachtranscript

_*Dataonfile.

RelatedProducts

SequencingchallengingRNAsamples?HowaboutDNA?CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:

KAPAhgDNAQuantificationandQCKit

KAPALibraryAmplificationKits

KAPAHyperPrepKits

KAPALibraryQuantificationKits

Applications:

Applications

Geneexpression
Singlenucleotidevariation(SNV)discovery
Post-transcriptionalSNVs
Fusiongeneidentification
Targetedtranscriptome
Wholetranscriptome

KitSpecificationsandContents/Storage:

KitSpecificationsandContents/Storage

Kitscanbestoredforupto8 months at-20˚C. KAPAmRNACaptureBeadscanbestoredforupto8months at-4˚C.

Components

mRNA_Component Chart

Specifications

CompatIBLePlatform

IlluminaHiSeq,NextSeq,MiSeqandGAIIx

StartingMaterial

TotalRNA,mRNA,orribosomaldepletedRNA

InputAmount

StrandedmRNA-SeqKits:100ng–4µgStrandedRNA-SeqKits:10ng–400ng

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