产品资料

Kapa biosystems/KAPA mRNA HyperPrep Kits/KK8701/12 adapters x 40 µl each

Description:

KAPASingle-IndexedAdapterKit,SetA(30µM)

KAPAmRNAHyperPrepKits

Single-DayRNA

TheKAPAmRNAHyperPrepKitsutilizenovelchemistrythatenablesthecombinationofenzymaticstepsandfewerreactionpurifications,resultinginatrulystreamlinedsolutionforthepreparationofhigh-qualitymRNA-seqlibraries.mRNAcapturebeadsareusedpriortolibrarypreparation,whichenrichesformaturemRNAovernon-polyadenylatedspecies,suchasribosomal,precursor,andnoncodingRNAs.

Thestrand-specificworkflowisflexIBLe–supportinglibraryconstructionfromlower-inputamountsanddegradedsamples.KitscontainallreagentsrequiredformRNAcaptureandlibrarypreparation,withtheexceptionofKAPAAdapters(availableseparately).Benefitsinclude:

single-daylibraryconstruction,inclusiveofmRNAcapture
reducedhands-onandoveralltimethroughfewerenzymaticandreactioncleanups
flexibleinputof50ng–1µgtotalRNA*
maintainover99%strandspecificity*
KAPAPureBeadsincludedforreactionpurifications

KAPARNAHyperPrepKits

KAPARNAHyperPrepKitsleveragethesameCDNAsynthesisandlibrarypreparationworkflowimprovementsasoutlinedfortheKAPAmRNAHyperPrepKits.KAPARNAHyperPrepKitsincludeallenzymesandbuffersrequiredforcDNAlibrarypreparation,butdonotcontainmRNAcapturereagentsorKAPAAdapters.Kitscanbeusedtopreparelibrariesfrom1ng–100ngoftotal,poly(A)-selected,orrRNA-depletedRNA.

NEW!KAPADual-IndexedAdapterKitsarenowavailable. FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapter andBeadCalculator.

DownloadourAdapterandBeadCalculator

^*Dataonfile.
ForResearchUseOnly.Notforuseindiagnosticprocedures.

ProductHighlights

The KAPA mRNA HyperPrep Kit results in a reduction in the total number of reads wasted due to both PCR duplicates and alignment to rRNA loci in comparison to the TruSeq and NEBNext kits. Libraries were generated in quadruplicate with 50 ng of high-quality Universal Human Reference (UHR) RNA using the manufacturers’ standard recommendations per workflow, where possible. Sequencing was performed using an Illumina HiSeq® 2500 in High Output mode with v4 chemistry and 2 x 100 bp read length. Reads aligning to rRNA were removed and paired reads were randomly subsampled to 14M for comparative analyses, including marked duplicates. Data on file.

With an equivalent amount of sequencing, more genes and unique transcripts are identified using the KAPA mRNA HyperPrep Kit in comparison to the TruSeq and NEBNext kits. Libraries were generated in quadruplicate with 50 ng of high-quality UHR RNA using the manufacturers’ standard recommendations per workflow, where possible. Sequencing was performed using an Illumina HiSeq® 2500 in High Output mode with v4 chemistry and 2 x 100 bp read length. Reads aligning to rRNA were removed and paired reads were randomly subsampled to 14M for comparative analyses, including marked duplicates. Data on file.

PrevNext

Sequencewhatmatters

WastefewerreadsduetothecombinationofrRNAcarryoverandPCRduplicates
Identifymoreuniquetranscriptsandgeneswithequivalentsequencing

For the top 1000 transcripts, the KAPA mRNA Hyper Prep Kit results in more even coverage across transcript lengths, as assessed by both a normalized coverage plot and coverage coefficient of variation (CV), in comparison to competitor workflows. Libraries were generated with 50 ng of high-quality UHR RNA using the manufacturers’ standard recommendations per workflow, where possible. Sequencing was performed using an Illumina HiSeq® 2500 in High Output mode with v4 chemistry and 2 x 100 bp read length. Reads aligning to rRNA were removed and paired reads were randomly subsampled to 14M for comparative analyses, including marked duplicates. Data on file.

Improved coverage of GC-rich regions (outlined in red) of the YBX1 (rRNA depletion) and SLC2A4RG (mRNA capture) genes, along with improved splice junction and antisense transcript detection was demonstrated using the KAPA mRNA Hyper Prep Kit in comparison to competitor workflows. Libraries were generated with 50 ng of high-quality UHR RNA using the manufacturers’ standard recommendations per workflow, where possible. Sequencing was performed using an Illumina HiSeq® 2500 in High Output mode with v4 chemistry and 2 x 100 bp read length. Reads aligning to rRNA were removed and paired reads were randomly subsampled to 14M for comparative analyses, including marked duplicates. Data on file.

PrevNext

Achievesuperiorcoverageuniformity

Obtainmoreuniformdistributionofreadsacrosstranscripts
ImprovecoverageofdifficultGC-richtranscripts

RelatedProducts

Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:

KAPARNAHyperPrepKitswithRiboErase

KAPAStrandedmRNA-SeqKits

KAPALibraryAmplificationKits

KAPAHyperPrepKits

KAPALibraryQuantificationKits

Applications:

Applications

Geneexpressionanalysis
Detectionofgenefusions,isoforms,andotherstructuralvariants
Noveltranscriptidentification,includingnoncodingtranscripts
SNVdiscovery
Wholetranscriptome(KAPARNAHyperPrepKitsonly)
Targetedtranscriptome(KAPARNAHyperPrepKitsonly)

KitSpecificationsandContents/Storage:

KitSpecificationsandContents/Storage

EnzymesandbuffersforrRNAdepletion,cDNAsynthesis,andlibrarypreparationcanbestoredforupto10monthsat-20°C.mRNAcapturereagentsandKAPAPureBeadscanbestoredforupto10monthsat4°C.(USonly)

KitsincludereagentsforRNAfragmentation,cDNAsynthesis,andlibrarypreparation.KitswithreagentsformRNAcapturearealsoavailable.

RNAHyper_Component Chart

Specifications

CompatibilityPlatform

IlluminaHiSeq,NextSeq,andMiSeq

LibraryType

RNA

StartingMaterial

RNAHyperPrepKit:High-qualitytotalRNA,mRNA,orrRNA-depletedRNA;mRNAHyperPrepKit:High-qualitytotalRNA