KAPALibraryQuantificationKitscontainallthereagentsneededfortheaccurate,reliableandreproducIBLeqPCR-basedquantificationofNGSlibrariespriortopoolingforcaptureorflowcellamplification.KitscontainKAPASYBR®FASTqPCRMasterMix,optimizedforhigh-performanceSYBRGreenI-basedqPCR.TheengineeredKAPASYBRFASTDNAPolymerasecontainedintheMasterMixamplifiesGC-andAT-richDNAfragmentsofdifferentlengthswithsimilarefficiency,makingittheonlyqPCRMasterMixcapableofaccurateqPCR-basedquantificationofNGSlibraries.*Thepre-dilutedsetofDNAStandardscontainedineachkitiscarefullyquality-controlledtoensurelot-to-lotconsistencyandavoiddatadriftovertime.OptimizedPrimerPremixesensureoptimalPCRefficiency.
KitswithDNAStandardsandPrimerPremixesforIllumina® andIonTorrent™sequencers areavailable.Pleasereferencethe InstrumentCompatibilityChart forguidanceoncompatibleplatforms.
NEW!ImproveexperimentaldesignswithourTechnicalGuideforIlluminaPlatforms
*Dataonfile.
ForResearchUseOnly.Notforuseindiagnosticprocedures.
*Dataonfile.
Normalizinglibrariesforpoolingandclusteramplification?CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:
Kitscanbestoredforupto12months at-20˚C.
CompletekitsincludeKAPASYBRFASTqPCRMasterMix(2X),PrimerPremix(10X)andasetof6DNAStandards.ReagentsforqPCR(KAPASYBRFASTqPCRMasterMixandPrimerPremix)andDNAStandards(1–6)arealsosoldseparately.Wherenoted,MasterMixescontaininstrument-specificreferencedyes,whiletheUniversalkitincludesROXHighandROXLow(both50X)separately.
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn