Kapa biosystems/KAPA Library Quantification Kits/KK4857/500 x 20 µL reactions

Description:

Complete kit (optimized for Roche® LightCycler 480)

KAPA Library Quantification Kits

for Next-Generation Sequencing

Our standards don’t change. Neither should yours.

KAPA Library Quantification Kits contain all the reagents needed for the accurate, reliable and reproducible qPCR-based quantification of NGS libraries prior to pooling for capture or flow cell amplification. Kits contain KAPA SYBR® FAST qPCR Master Mix, optimized for high-performance SYBR Green I-based qPCR. The engineered KAPA SYBR FAST DNA Polymerase contained in the Master Mix amplifies GC- and AT-rich DNA fragments of different lengths with similar efficiency, making it the only qPCR Master Mix capable of accurate qPCR-based quantification of NGS libraries.* The pre-diluted set of DNA Standards contained in each kit is carefully quality-controlled to ensure lot-to-lot consistency and avoid data drift over time. Optimized Primer Premixes ensure optimal PCR efficiency.

Kits with DNA Standards and Primer Premixes for Illumina® and Ion Torrent™ sequencers are available. Please reference the Instrument Compatibility Chart for guidance on compatible platforms.

NEW! Improve experimental designs with our Technical Guide for Illumina Platforms


*Data on file.

For Research Use Only. Not for use in diagnostic procedures.

Product Highlights

Accurately quantify PCR-competent sequencing templates

  • qPCR only “counts” those library molecules that can be sequenced
  • More consistent cluster densities or template-to-bead ratios enable optimal utilization of sequencing resources
Equimolar pooling of indexed libraries. Eleven indexed Illumina TruSeq libraries were quantified by qPCR using the KAPA Library Quantification Kit, and then combined to achieve equal final concentrations in two separate pools for multiplexed sequencing on different flow-cell lanes. The eleven libraries ranged ~11-fold in concentration from 0.67 pM to 7.65 pM, while representation of each index varied between 90% and 127% of expected assigned reads per lane. Data on file.

Improve pooling for multiplexed sequencing

  • Library concentrations determined by qPCR are more reliable compared to other methods*
  • The engineered KAPA SYBR FAST qPCR Master Mix enables accurate pooling of libraries with extreme GC-contents or longer inserts*
Nine human DNA libraries and two microbial DNA libraries (Rhodococcus sp. ~70% GC and Staphylococcus sp. ~35% GC) were used to compare quantification results obtained with distinct lots (”Lot 1” and “Lot 2”), and distinct sets of reagents from the same lot (”Set 1” and “Set 2”) of KAPA Library Quantification Kits for Illumina platforms. Data on file.

Eliminate drift with carefully quality-controlled DNA Standards

  • Prediluted DNA Standards eliminate quantification errors associated with preparation of standard curves using in-house standards*
  • KAPA DNA Standards undergo strict quality control to ensure lot-to-lot consistency and eliminate data drift over time
KAPA Library Quantification methods (96- and 384-well) format are available for the Sciclone NGS and NGSx workstation (PerkinElmer) and other instruments used in high-throughput NGS sample preparation pipelines.

Improve throughput with automation

  • Library quantification assay is compatible with 96- and 384-well format
  • Library dilution, reaction setup and data analysis can be automated for HTP pipelines
  • Learn More about KAPA Automated Solutions

*Data on file.

Related Products

Normalizing libraries for pooling and cluster amplification? Check out these Kapa NGS products to improve your workflow and results:

Sample QC

KAPA hgDNA Quantification and QC Kit

Library Amplification

KAPA Library Amplification Kits

Library Preparation

KAPA Hyper Prep Kits

KAPA Stranded mRNA-Seq Kits

KAPA Stranded mRNA-Seq Kits

Applications:

Applications
  • Quantification of libraries prior to pooling for multiplexed sequencing
  • Quantification of individual libraries or library pools prior to cluster amplification or emPCR
  • Quantification of libraries prior to and after hybridization capture
  • Quality control, optimization and troubleshooting of library construction processes or workflows

Kit Specifications and Contents / Storage:

Kit Specifications and Contents / Storage

Kits can be stored for up to 12 months at -20˚C.

Complete kits include KAPA SYBR FAST qPCR Master Mix (2X), Primer Premix (10X) and a set of 6 DNA Standards. Reagents for qPCR (KAPA SYBR FAST qPCR Master Mix and Primer Premix) and DNA Standards (1–6) are also sold separately. Where noted, Master Mixes contain instrument-specific reference dyes, while the Universal kit includes ROX High and ROX Low (both 50X) separately.

Components–Illumina® Platform

Components–Ion Torrent™ Platform

LQK-Ion_Component Chart

Specifications

Spec
Description
Compatible Platforms
Illumina HiSeq, NextSeq, MiSeq, and GAIIxIon Torrent Proton and PGM454 GS FLX+ and GS JuniorSOLiD 5500 series
Library Type
Any
Starting Material
Any NGS library ready for cluster amplification or emPCR
Standard curve concentration range
20–0.0002 pM for Illumina libraries83–0.00083 pM for Ion Torrent and 454 libraries10–0.0001 pg/µL for SOLiD libraries
Sequencing Applications
Whole Genome Sequencing Whole Exome Sequencing Targeted Sequencing (custom panels)RNA-SeqChIP-SeqAmplicon Sequencing Methyl-Seq

新闻动态
行业前沿
技术文章
最新产品

188进口试剂采购网 www.188bio.cn -中国试剂网,试剂网,化学试剂网,国药试剂,抗体公司,试剂公司,试剂盒公司,苏州试剂公司,北京化学试剂公司,天津化学试剂,试剂商城,试剂代理,流式抗体 细胞库查询 sitemap