Product Description
The CleanPlex® Dual-Indexed PCR Primers for Illumina® are high-quality ready-to-use PCR primers for Illumina library construction. They are compatible and designed for use with all CleanPlex Panels to construct targeted libraries for sequencing on an Illumina NGS platform. The index set comprises of i5 primers and i7 primers that form combinatorial indexing of samples for multiplexing samples per sequencing run. Samples with unique i5 and i7 combinations can be pooled and sequenced then demultiplexed for analysis.
| CleanPlex Dual-Indexed PCR Primers for Illumina | ||||
| Description | SKU | Reactions | Available combinations/Kit | Format |
| CleanPlex® Dual-Indexed PCR Primers for Illumina® Set T (3 x 3 indexes, 9 reactions) | 716021 | 9 | 3 x 3, 9 combinations (Random selection of Set A indexes) | 6 Tubes |
| CleanPlex® Dual-Indexed PCR Primers for Illumina Set A* (12 x 8 indexes, 96 reactions) | 716006 | 96 | 12 x 8, 96 combinations | 20 Tubes |
| CleanPlex® Dual-Indexed PCR Primers for Illumina Set A (12 x 8 indexes, 384 reactions) | 716017 | 384 | 12 x 8, 96 combinations | 20 Tubes |
| CleanPlex® Dual-Indexed PCR Primers for Illumina Set B* (12 x 8 indexes, 96 reactions) | 716018 | 96 | 12 x 8, 96 combinations | 20 Tubes |
| CleanPlex® Dual-Indexed PCR Primers for Illumina Set B (12 x 8 indexes, 384 reactions) | 716019 | 384 | 12 x 8, 96 combinations | 20 Tubes |
| CleanPlex® Dual-Indexed PCR Primers for Illumina Set L (56 x 48 indexes, 2688 reactions) | 716020 | 2688 | 56 x 48 , 2688 combinations | 104 tubes |
*Sets A and B’s index sequences are mutually exclusive. Can be combined for additional multiplexing needs.
Primer Sequences:
i5 Indexed Primers
5’-AATGATACGGCGACCACCGAGATCTACACXXXXXXXXACACTCTTTCCCTACACGACGCTCTTCCGATCT-3′
i7 Indexed Primers
5’-CAAGCAGAAGACGGCATACGAGATXXXXXXXXGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT-3’
Notes:
- Please refer to CleanPlex NGS Panel User Guide for instructions on using CleanPlex Dual-Indexed PCR Primers for Illumina
- CleanPlex Dual-Indexed PCR primers for Illumina Set A1 and A2 have been discontinued and is no longer available for ordering.
- This product listing is for indexes delivered in single tube format, not 96 well plated format.
- Set A and Set B index sequences are mutually exclusive and can be sequenced together for 192 sample multiplexing or combined to created 384 available combinations.
Storage Temperature:
Store at -20°C
For Research Use Only. Not for use in diagnostic procedures.
