Product Description
The CleanPlex® Unique Dual-Indexed (UDI) PCR Primers for Illumina® are high-quality ready-to-use PCR primers for Illumina library construction. They are compatible and designed for use with all CleanPlex Panels to construct targeted libraries for sequencing on an Illumina NGS platform. The index set comprises of uniquely pooled i5 and i7 index primer pools for unique dual multiplexing of samples per sequencing run. Each set of UDI primer pool only contains one instance of each i5 and each i7 to mitigate index hopping concerned on Illumina sequencing platforms with patterned flowcells. Samples indexes with each i5 and i7 pair can be pooled and sequenced then demultiplexed for analysis.
| CleanPlex Unique Dual-Indexed (UDI) PCR Primers for Illumina | ||||
| Description | SKU | Reactions | Available Unique Indexes/Kit | Format |
| CleanPlex® Unique Dual-Indexed PCR Primers for Illumina Set T8 (8 indexes, 8 reactions) | 716015 (Available upon request) | 8 | 8 Unique Index Pairs, (Random selection of Set A or Set B indexes) | 8 Tubes |
| CleanPlex® Unique Dual-Indexed PCR Primers for Illumina Set A * (16 indexes, 32 reactions) | 716011 | 32 | 16 Unique Index pairs | 16 Tubes |
| CleanPlex® Unique Dual-Indexed PCR Primers for Illumina Set A (16 indexes, 96 reactions) | 716012 | 96 | 16 Unique Index pairs | 16 Tubes |
| CleanPlex® Unique Dual-Indexed PCR Primers for Illumina Set B* (16 indexes, 32 reactions) | 716013 | 32 | 16 Unique Index pairs | 16 Tubes |
| CleanPlex® Unique Dual-Indexed PCR Primers for Illumina Set B (16 indexes, 96 reactions) | 716014 | 96 | 16 Unique Index pairs | 16 Tubes |
*Sets A and B’s index sequences are mutually exclusive. Can be combined for a total of 32 available UDI options.
Primer Sequences:
i5 Indexed Primers
5’-AATGATACGGCGACCACCGAGATCTACACXXXXXXXXACACTCTTTCCCTACACGACGCTCTTCCGATCT-3′
i7 Indexed Primers
5’-CAAGCAGAAGACGGCATACGAGATXXXXXXXXGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT-3’
Notes:
- Please refer to CleanPlex UMI NGS Panel User Guide for instructions on using CleanPlex Dual-Indexed PCR Primers for Illumina
- This product listing is for indexes delivered in single tube format, not 96 well plated format.
- Set A and Set B index sequences are mutually exclusive and can be sequenced together for a total of 32 sample multiplexing
Storage Temperature:
Store at -20°C
For Research Use Only. Not for use in diagnostic procedures.
