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MCLAB/Lambda Exonuclease/LE-200/20,000 units

LamBDaExonucleasecatalyzesthehydrolysisofinorganicpyrophosphatetoformorthophosphate.

Description:
LambdaExonucleaseisahighlyprocessiveexonucleasewhichselectivelydegradesthe5'-phosphorylatedstrandofdouble-strandedDNAviathestepwise5'to3'releaseofmononucleotidesfromduplexDNA.LambdaExonucleaseisinactiveagainst5'-hydroxyltermini(1),andwillnotinitiateexcisionatanickorgap(2),thoughitwilldegradea5'-overhangingtailfromduplexDNAatagreatlyreducedrate.

SpecificActivity:100,000U/mg

Application:
Catalyzestheremovalof5'-mononucleotidesfromduplexDNA.

Source:
PurifiedfromastrainofE.colithatoverexpressestheexonucleasegenefrombacteriophageLambda.

Suppliedin:
25mMTris-HCl
50mMNaCl
1.0mMDTT
0.1%mMEDTA
50%Glycerol
pH7.5@25°C

Suppliedwith:
10xLambdaExoReactionBuffer

10xLambdaExoReactionBuffer:
670mMGlycine
25mMMgCl2
500μg/mlBSA
pH9.4@25°C

UnitDefinition:
Oneunitisdefinedastheamountofenzymerequiredtoproduce10nmolofacid-solubledeoxyribonucleotidefromdouble-strandedsubstratesin30minutesat37°C.

RecommendedStorageCondition:-20°C

References:
1.Ausubel,F.M.,etal.(1987)CurrentProtocolsinMolecularBIOLOGy(JohnWileyandSons,Inc.)
2.Little,J.W.(1981)GeneAmp.Anal.,2,135-145

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